Enterococcus faecalis and/or enterococcus faecium antigen
AssigneeUniversitatsklinikum Freiburg
InventorsJOHANNES HÜBNER, OTTO HOLST, CHRISTIAN THEILACKER, ZBIGNIEW KACZYNSKI
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Abstract
The present invention generally relates to the field of detecting and preventing infectious diseases caused by Enterococcus faecalis and/or Enterococcus faecium. More specifically, the invention relates to an Enterococcus faecalis and/or Enterococcus faecium antigen which comprises at least one unit having the following general formula:
Claims20
A method of producing an antibody that specifically binds an antigen, wherein the antigen comprises at least one unit having the following general formula:
- wherein:
- R1 are independently of one another selected from the group consisting of H, OH, OCH3, OAc, OCnHm, OFo, OAcyl, SH, SCH3, SCnHm, SFo, SAc, SAcyl, NH2, NHCH3, NHCnHm, NHFo, NHAc, NHAcyl, PO2(OR2)2, F, Cl, Br and I,
- Fo is formyl, R2 is independently selected from the group consisting of H, CnHm, CnHm-1NHR3 and CnHm-1N(CH3)3,
- R3 is independently selected from the group consisting of H, Fo, Ac and acyl,
- each X is independently of one another selected from the group consisting of O, S, CH2, NH and POOR4,
- R4 is H, CnHm, CnHm-1NHR3 and CnHm-1N(CH3)3,
- each Y is independently of one another selected from the group consisting of O, S, CH2 and HPO4,
- CA is independently of one another selected from the group consisting of C1-C6-acyl radicals, in particular hydroxyacyl radicals, preferably lactyl, H, OH, OCH3, OAc, OCnHm, OFo, OAcyl, SH, SCH3, SCnHm, SFo, SAc, SAcyl, NH2, NHCH3, NHCnHm, NHFo, NHAc, NHAcyl, PO2OR2, F, Cl, Br and I,
- Fo is formyl, R2 is independently selected from the group consisting of H, OCnHm, OCnHm-1NHR3 and OCnHm-1N(CH3)3,
- R3 is independently selected from the group consisting of H, Fo, Ac and acyl,
- m=2n+1, and
- n is selected from the set of natural numbers from 1 to 10,
- and wherein the method comprises administering the antigen to an animal in an amount sufficient for inducing the production of the antibody that specifically binds the antigen.
A method of preventing an Enterococcal infection in an animal, comprising administering to the animal an antigen in an amount sufficient to induce the production of antibodies that specifically bind to the antigen, wherein the antigen comprises at least one unit having the following general formula:
- wherein:
- R1 are independently of one another selected from the group consisting of H, OH, OCH3, OAc, OCnHm, OFo, OAcyl, SH, SCH3, SCnHm, SFo, SAc, SAcyl, NH2, NHCH3, NHCnHm, NHFo, NHAc, NHAcyl, PO2(OR2)2, F, Cl, Br and I,
- Fo is formyl, R2 is independently selected from the group consisting of H, CnHm, CnHm-1NHR3 and CnHm-1N(CH3)3,
- R3 is independently selected from the group consisting of H, Fo, Ac and acyl,
- each X is independently of one another selected from the group consisting of O, S, CH2, NH and POOR4,
- R4 is H, CnHm, CnHm-1NHR3 and CnHm-1N(CH3)3,
- each Y is independently of one another selected from the group consisting of O, S, CH2 and HPO4,
- CA is independently of one another selected from the group consisting of C1-C6-acyl radicals, in particular hydroxyacyl radicals, preferably lactyl, H, OH, OCH3, OAc, OCnHm, OFo, OAcyl, SH, SCH3, SCnHm, SFo, SAc, SAcyl, NH2, NHCH3, NHCnHm, NHFo, NHAc, NHAcyl, PO2OR2, F, Cl, Br and I,
- Fo is formyl, R2 is independently selected from the group consisting of H, OCnHm, OCnHm-1NHR3 and OCnHm-1N(CH3)3,
- R3 is independently selected from the group consisting of H, Fo, Ac and acyl,
- m=2n+1, and
- n is selected from the set of natural numbers from 1 to 10.
A method of preventing an Enterococcal infection in an animal, comprising administering to the animal an antibody or a fragment thereof that specifically binds an antigen, wherein the antigen comprises at least one unit having the following general formula:
- wherein:
- R1 are independently of one another selected from the group consisting of H, OH, OCH3, OAc, OCrHm, OFo, OAcyl, SH, SCH3, SCnHm, SFo, SAC, SAcyl, NH2, NHCH3, NHCnHm, NHFo, NHAc, NHAcyl, PO2(OR2)2, F, Cl, Br and I,
- Fo is formyl, R2 is independently selected from the group consisting of H, CnHm, CnHm-1NHR3 and CnHm-1N(CH3)3,
- R3 is independently selected from the group consisting of H, Fo, Ac and acyl,
- each X is independently of one another selected from the group consisting of O, S, CH2, NH and POOR4,
- R4 is H, CnHm, CmHm-1NHR3 and CnHm-1N(CH3)3,
- each Y is independently of one another selected from the group consisting of O, S, CH2 and HPO4,
- CA is independently of one another selected from the group consisting of C1-C6-acyl radicals, in particular hydroxyacyl radicals, preferably lactyl, H, OH, OCH3, OAc, OCnHm, OFo, OAcyl, SH, SCH3, SCnHm, SFo, SAC, SAcyl, NH2, NHCH3, NHCnHm, NHFo, NHAc, NHAcyl, PO2OR2, F, Cl, Br and I,
- Fo is formyl, R2 is independently selected from the group consisting of H, OCnHm, OCnHm-1NHR3 and OCnHm-1N(CH3)3, R3 is independently selected from the group consisting of H, Fo, Ac and acyl,
- m=2n+1, and
- n is selected from the set of natural numbers from 1 to 10.
Dependencies are inferred from the claim text.