Hydrated and anhydrous polymorphs of 2'-o-fucosyllactose and their production methods
AssigneeBASF SE
InventorsGiovanni Cipolletti, Gessica Laudati, Liana Salsini, Michael Puhl
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Abstract
This invention describes new hydrated and anhydrous polymorphs of 2′-0-ficosyllactose (2′FL): Polymorph A 2′FL-3/2H2O, Polymorph B 2TL-5/2H2O and anhydrous Polymorph C. There is also a description of the methods for obtaining them, and of a new method for preparing Polymorph I already known in the literature.
Claims22
-16. (canceled)
2′-O-Fucosyllactose (2′FL) in anhydrous crystalline form of polymorph C, wherein said polymorph C has characteristic XRPD peaks at 17.58±0.20, 17.44±0.20, 10.63±0.20 2Θ.
A method for preparing the polymorph I, having an X-ray powder diffraction with characteristic peaks at angles 2Θ 21.34±0.20°, 20.92±0.20°, 18.37±0.20°, 16.70±0.20°, 9.91±0.20°, 13.13±0.20°, 7.87±0.20° and 8.90±0.20°, said method comprising the crystallization from glacial acetic acid.
-23. (canceled)
A method for obtaining crystalline 2′-O-Fucosyllactose (2′FL) anhydrate in form of polymorph C with molecular formula C18H32O15, the method comprising:
- providing a mixture comprising (i) a C1-C3 alcohol or mixture thereof and (ii) a 2′FL hydrate in form of polymorph A with molecular formula C18H32O15.nH2O wherein n is 3/2;
- and
- warming the mixture at a temperature in range of 30° C. to 70° C. for 1 hr to 24 hr, thereby crystallizing the 2′FL and forming 2′FL anhydrate in form of polymorph C crystals suspended in the mixture.
Dependencies are inferred from the claim text.