Substances Containing AuCs and Preparation Method and Use Thereof
AssigneeShenzhen Profound-View Pharma Tech Co., Ltd
InventorTaolei SUN
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Abstract
Disclosed are a pharmaceutical use of a gold cluster and a substance containing the gold cluster and the preparation method and use thereof. The gold cluster and substance containing the gold cluster can inhibit the aggregation of Aβ and α-syn, has excellent effects on the levels of cell models and animal models, and can be used to prepare drugs for preventing and treating Alzheimer's disease and/or Parkinson's disease.
Claims30
A substance containing gold clusters (AuCs), said substance comprising:
- AuCs;
- and
- a ligand Y coating the AuCs externally.
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A method for preparing an AuCs-containing substance, said method comprising the following steps:
- (1) dissolving HAuCl4 in a solvent to obtain a solution A with a 0.01˜0.03M concentration of HAuCl4;
- wherein the solvent is selected from the group consisting of methanol, water, ethanol, n-propanol and ethyl acetate;
- (2) dissolving ligand Y in a solvent to obtain a solution B with a 0.01˜0.18M concentration of the ligand Y;
- (3) mixing the solution A in step (1) with the solution B in step (2) to obtain a reaction solution, wherein the mole ratio of HAuCl4 and the ligand Y in the reaction solution is 1:(0.01˜100), wherein the reaction solution is stirred in an ice bath for 0.1˜48 h, wherein adding into the reaction solution 0.025˜0.8M solution of NaBH4 in a solvent selected from the group consisting of water, ethanol, and methanol, and wherein the reaction solution is stirred in an ice bath for 0.1˜12 h, wherein the mole ratio of NaBH4 and the ligand Y is 1:(0.01˜100);
- (4) centrifuging the reaction solution in step (3) at 8000˜17500 r/min for 10˜100 min, thereby precipitate of AuCs with different average particle sizes is obtained;
- (5) dissolving the precipitate of AuCs with different average particle sizes obtained in step (4) in water, wherein the dissolved precipitate of AuCs is put in a dialysis bag and is-dialyzed in water at room temperature for 1˜7 days;
- and
- (6) freeze-drying the AuCs solution in the dialysis bag for 12˜24 h, thereby the AuCs-containing substance is obtained.
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Dependencies are inferred from the claim text.