Hydroxyalkylated polyacrylamide surface coatings for in situ synthesis of dna arrays
AssigneeCentrillon Technology Holdings Corporation
InventorGlenn MCGALL
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Abstract
The present disclosure relates to processes for derivatizing a surface of a substrate with a covalently bonded thin film of hydroxalkylated poly(acrylamide) as a platform for the synthesis of a biomolecule array. These processes can also be used to prepare a surface of a substrate for an in situ solid-phase synthesis of biomolecule array.
Claims28
A solid support, comprising covalently surface-bonded polymers comprising a compound of Formula I:
- wherein R1 is independently selected from the group comprising:
- with the proviso that at least one R1 is
- T1 is absent, H, C1-C6 alkyl, or a first initiator residue;
- T2 is absent, H, C1-C6 alkyl or a second initiator residue;
- R2 is independently H, —CH3, or —CH2OCH3;
- R3 and R4, in each occurrence, are independently H or —CH3;
- Capture Probe comprises at least one molecule selected from the group consisting of peptide, protein, glycosylated protein, glycoconjugate, aptomer, carbohydrate, polynucleotide, oligonucleotide and polypeptide;
- x is independently an integer from 1 to 20;
- y is independently an integer from 1 to 20;
- z is independently an integer from 2 to 200;
- p is independently an integer from 0 to 20;
- q is independently an integer from 0 to 20;
- a is an integer from 1 to 5;
- b is an integer from 0 to 10;
- c is an integer from 1 to 5;
- and
- d is an integer from 0 to 10.
The solid support of claim 6, wherein the Capture Probe is DNA.
A solid support, comprising covalently surface-bonded polymers comprising a compound of Formula I:
- wherein R1 is independently selected from the group consisting of:
- T1 is absent, H, C1-C6 alkyl, or a first initiator residue;
- T2 is absent, H, C1-C6 alkyl or a second initiator residue;
- R2 is independently H, —CH3, or —CH2OCH3;
- R3 and R4, in each occurrence, are independently H or —CH3;
- x is independently an integer from 1 to 20;
- y is independently an integer from 1 to 20;
- z is independently an integer from 2 to 200;
- p is independently an integer from 0 to 20;
- q is independently an integer from 0 to 20;
- a is an integer from 1 to 5;
- b is an integer from 0 to 10;
- c is an integer from 1 to 5;
- and
- d is an integer from 0 to 10.
A method of derivatizing a surface of a substrate, comprising:
- (a) providing a substrate having a surface comprising a plurality of first amino groups;
- (b) reacting a first set of a plurality of reactive groups of a first reagent with a set of the plurality of the first amino groups, thereby forming a covalently surface-bonded film;
- and
- (c) reacting a second set of the plurality of the reactive groups of the first reagent with a second reagent comprising a second amino group and a hydroxyalkyl-functionalized group, thereby forming a hydroxyalkylated surface-bonded film.
Dependencies are inferred from the claim text.