Momp telonanoparticles, and related compositions, methods and systems
AssigneesLawrence Livermore National Security, LLC, Synthetic Genomics, Inc.
InventorsMatthew A. COLEMAN, Nicholas O. FISCHER, Amy RASLEY, Craig D. BLANCHETTE, Todd PETERSON
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Abstract
A telodendrimer-nanolipoprotein particle (t-NLP), comprising one or more membrane forming lipids, one or more telodendrimers, and a scaffold protein and a Chlamydia major outer membrane protein (MOMP) comprising a MOMP hydrophobic region, and related compositions methods and systems.
Claims62
A telodendrimer-nanolipoprotein particle (t-NLP), comprising one or more membrane forming lipids,
- one or more telodendrimers, and
- a scaffold protein and a Chlamydia major outer membrane protein (MOMP) and/or a fragment thereof comprising a MOMP hydrophobic region,
- wherein the one or more membrane forming lipids are arranged in a discoidal membrane lipid bilayer stabilized by the scaffold protein and the one or more telodendrimers, with the membrane lipid bilayer attaching the MOMP and/or the fragment thereof through interaction of the MOMP hydrophobic region with the membrane lipid bilayer.
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A method to provide a telodendrimer-nanolipoprotein particle presenting a Chlamydia major outer membrane proteins (MOMP), the method comprising
- providing one or more membrane forming lipids, one or more telodendrimers, a polynucleotide coding for the MOMP and a polynucleotide coding for a scaffold protein;
- mixing the one or more membrane forming lipids and the one or more telodendrimers to provide a lipid-telodendrimer mixture;
- mixing lipid-telodendrimer mixture with, the polynucleotide coding for the MOMP and the polynucleotide coding for the scaffold protein with an in vitro cell free translation system to provide a single reaction mixture;
- and
- translating the polynucleotide within the single reaction mixture via the in vitro cell free translation system,
- wherein the mixing and translating are performed to allow self-assembly of the scaffold protein, the one or more membrane forming lipids and the one or more telodendrimers into a nanolipoprotein particle, the nanolipoprotein particle comprising the MOMP within a discoidal membrane lipid bilayer formed by the one or more membrane forming lipids and stabilized by the scaffold protein, the membrane lipid bilayer attaching the MOMP through interaction of a hydrophobic region of the MOMP with the membrane lipid bilayer.
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A method to provide a telodendrimer-nanolipoprotein particle presenting a Chlamydia major outer membrane proteins (MOMP), the method comprising
- providing one or more membrane forming lipids, one or more telodendrimers, a scaffold protein and a polynucleotide coding for the MOMP;
- mixing the one or more membrane forming lipids and the one or more telodendrimers to provide a lipid-telodendrimer mixture;
- mixing lipid-telodendrimer mixture with the scaffold protein and the polynucleotide coding for the MOMP with an in vitro cell free translation system to provide a single reaction mixture;
- and
- translating the polynucleotide within the single reaction mixture via the in vitro cell free translation system,
- wherein the mixing and translating are performed to allow self-assembly of the scaffold protein, the one or more membrane forming lipids and the one or more telodendrimers into a nanolipoprotein particle, the nanolipoprotein particle comprising the MOMP within a discoidal membrane lipid bilayer formed by the one or more membrane forming lipids and stabilized by the scaffold protein, the membrane lipid bilayer attaching the MOMP through interaction of a hydrophobic region of the MOMP with the membrane lipid bilayer.
Dependencies are inferred from the claim text.