Peptides whose uptake in cells is controllable
AssigneeThe Regents of the University of California
InventorsRoger Tsien, Todd Aguilera, Emilia Olson, Tao Jiang, Quyen Nguyen
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Abstract
Disclosed herein, in certain embodiments, is a selective transport molecule with increased in vivo circulation. In some embodiments, a selective transport molecule disclosed herein has the formula (A-X-B-C)-M, wherein C is a cargo moiety; A is a peptide with a sequence comprising 5 to 9 consecutive acidic amino acids, wherein the amino acids are selected from: aspartates and glutamates; B is a peptide with a sequence comprising 5 to 20 consecutive basic amino acids; X is a linker; and M is a macromolecular carrier.
Claims15
-76. (canceled)
A molecule comprising a structure A-X-B-C,
- wherein:
- A is a peptide with (i) a sequence comprising a series of 5 to 9 acidic amino acids selected from:
- aspartates and glutamates, and (ii) an unsubstituted maleimide bound to the N-terminus of the peptide;
- B is a peptide with a sequence comprising a series of 5 to 20 basic amino acids;
- X is a cleavable linker selected from:
- PLGLAG (SEQ ID NO:1), PLGLAx (SEQ ID NO:2) wherein X is any amino acid, PLG-C(me)-AG (SEQ ID NO:3), ESPAYYTA (SEQ ID NO:4), RLQLKL (SEQ ID NO:5), and RLQLK(AC) (SEQ ID NO:6);
- and
- C is a therapeutic cargo selected from a chemotherapeutic agent, a radiation sensitizer, a pro-apoptotic agent, or an anti-apoptotic agent.
Dependencies are inferred from the claim text.