US 2020/0046820 A1 · VACCINE DIRECTED TO INDUCTION OF IMMUNE RESPONSE TO PROTEIN AND GLYCOLIPID ANTIGENS OF BACTERIAL CELLS THROUGH INTERACTION OF CD40L/CD40 RECEPTOR AXIS WITH COMPLEX OF GLYCOLIPID/CD1d RECEPTOR IN NKT CELLS AND IN DENDRITIC CELLS

Abstract

A combination of components to promote an innate and adaptive immune response comprising of a TAA/ecdCD40L vaccine and a complex between the CD1d receptor and an alpha galactosyl ceramide like glycolipid (AGCLGL), to activate NKT cells and activate the CD40 receptor on the DCs and increase the level of the adaptive immune response induced by the TAA/ecdCD40L vaccine to the TAA. The result and advantage of using both the TAA/ecdCD40L vaccine and the α-galactosylceramide-CD1d complex (or a related bacterial or other antigen related to α-galactosylceramide) to stimulate the immune response through the CD40L/CD40 axis on dendritic cells, is that the magnitude of the stimulation is robust and increased significantly more than additive—i.e. synergistically due to the interaction, cross-talk and/or cross-stimulation of the glycolipid-CD1d pathway and TAA/ecdCD40L pathway. As a result, a potent immune response is induced against lipid target antigens as well as protein target antigens.

Claims16

Dependent claims

A method for activating an individual's innate and adaptive immune response by administering to the individual first and second compositions,

(i) the first composition comprising an effective amount of a complex comprising an alpha galactosyl ceramide like glycolipid (AGCLGL) bound to the CD1d protein (AGCLGL-ecdCD1d) to promote activation of NKT cells and activation of an individual's dendritic cells, and T cell lymphocytes;
and
(ii) the second composition comprising an effective amount of a fusion protein comprising a target associated antigen fused to the ecd of the CD40L (TAA/CD40L) configured to promote activation of dendritic cells and activation and expansion of TAA specific cytotoxic CD8+ effector T cells and TAA B cells inducing the release of TAA specific antibodies;
(iii) wherein each of said first and second compositions is separately administered to the individual and subsequent to administering the first and second compositions, interaction of the first and second compositions is promoted through a CD40L/CD40 axis on dendritic cells.

1 more element

Dependencies are inferred from the claim text.

VACCINE DIRECTED TO INDUCTION OF IMMUNE RESPONSE TO PROTEIN AND GLYCOLIPID ANTIGENS OF BACTERIAL CELLS THROUGH INTERACTION OF CD40L/CD40 RECEPTOR AXIS WITH COMPLEX OF GLYCOLIPID/CD1d RECEPTOR IN NKT CELLS AND IN DENDRITIC CELLS

Life of this patent

VACCINE DIRECTED TO INDUCTION OF IMMUNE RESPONSE TO PROTEIN AND GLYCOLIPID ANTIGENS OF BACTERIAL CELLS THROUGH INTERACTION OF CD40L/CD40 RECEPTOR AXIS WITH COMPLEX OF GLYCOLIPID/CD1d RECEPTOR IN NKT CELLS AND IN DENDRITIC CELLS

Life of this patent

Abstract

Claims16