Methods and products for nucleic acid production and delivery

An in vivo method for treating dystrophic epidermolysis bullosa, comprising

• delivering a synthetic RNA encoding a gene-editing protein that targets a COL7 gene to a patient in need thereof and
• delivering a COL7 repair template to the patient,
• thereby editing the COL7 gene, wherein:
• the synthetic RNA and repair template are delivered to the patient's keratinocytes by administration to the epidermis and
• the gene-editing protein comprises a DNA-binding domain and a nuclease domain and causes a double-strand break in the COL7 gene of the patient's keratinocytes.

An in vivo method for treating epidermolysis bullosa, comprising

• delivering a synthetic RNA encoding a gene-editing protein that targets a COL7 gene to a patient in need thereof and
• inducing a single-strand or double-strand break in the COL7 gene of the patient's keratinocytes,
• thereby eliminating a mutation that is at least partially responsible for a disease phenotype, wherein:
• the synthetic RNA is delivered to the patient's keratinocytes by administration to the epidermis and
• the gene-editing protein comprises a DNA-binding domain and a nuclease domain.