Recombinant oncolytic viruses for cancer therapy
AssigneesUniversity Health Network, Children's Hospital of Eastern Ontario Research Institute Inc.
InventorsJudith Andrea MCCART, David Francis STOJDL, Tiffany Yun-Yee HO
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Abstract
The disclosure provides a recombinant oncolytic poxvirus including vaccinia virus comprising one or more inactivated immunomodulatory gene selected from NIL, K1L, K3L, A46R, and/or A52R, optionally further comprising inactivated TK and/or VGF genes. The disclosure also provides methods and for the use of these recombinant oncolytic poxvirus in oncolytic virotherapy. Also provided in the disclosure are vector constructs for generating recombinant oncolytic poxviruses including for example vaccinia viruses that have one or more inactivated immunomodulatory genes.
Claims27
A recombinant vector comprising:
- a) a left flanking portion of a poxvirus gene selected from N1L, K1L, K3L, A46R, and A52R gene, and
- b) a right flanking portion of said poxvirus gene,
- wherein the left flanking portion and the right flanking portion are operably linked to a detectable interrupter expression cassette
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A recombinant oncolytic poxvirus, optionally a recombinant oncolytic vaccinia virus or viral DNA thereof, comprising one or more inactivated poxvirus genes selected from N1L, K1L, K3L, A46R, and/or A52R, preferably selected from K1L, A46R and/or A52R, optionally further comprising an inactivated TK gene and/or one or more inactivated growth factor genes, optionally VGF genes.
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An isolated DNA molecule used for amplifying flanking regions of a poxvirus gene selected from N1L, K1L, K3L, A46R and A52R, optionally the isolated DNA molecule selected from SEQ ID NOs:1-20 or an isolated DNA molecule comprising 5′ and/or 3′ overhang and sequence of flanking portion of a poxvirus gene selected from N1L, K1L, K3L, A46R and A52R, wherein the sequence amplified using a primer pair, optionally the primer pair selected from SEQ ID NOs:1-20.
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Dependencies are inferred from the claim text.