Cryopreserved platelet compositions and methods for making
AssigneeCellphire, Inc.
InventorsBenjamin J. Kuhn, Stephen Edward Amos, Rafael Jorda
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Abstract
This disclosure relates to a composition that, in some embodiments, includes cryopreserved platelets; and a cryoprotectant, wherein the composition comprises less than 6% (v/v) of dimethyl sulfoxide (DMSO). In some embodiments, the composition is substantially free of dimethyl sulfoxide (DMSO).
Claims65
A composition comprising:
- cryopreserved platelets;
- and
- a cryoprotectant;
- wherein the composition comprises DMSO in the amount of about 0.1% (v/v) to about 5% (v/v).
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A method of making a composition comprising:
- cryopreserved platelets;
- and
- a cryoprotectant;
- wherein the composition comprises dimethyl sulfoxide (DMSO) in the amount of about 0.1% (v/v) to about 5% (v/v), the method comprising:
- obtaining platelets contained in a liquid medium;
- suspending the platelets in a suspension medium comprising a cryoprotectant, the suspension medium comprising dimethyl sulfoxide (DMSO) in the amount of about 0.1% (v/v) to about 5% (v/v);
- and
- subjecting the suspended platelets to a temperature sufficient to form the composition.
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The method of claim 86, wherein the suspension medium further comprises an organic solvent, wherein the organic solvent is present in an amount of about 0.1% to about 2% (v/v) of the composition.
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A method of making a composition comprising:
- cryopreserved platelets;
- and
- a cryoprotectant;
- wherein the composition comprises dimethyl sulfoxide (DMSO) in the amount of about 0.1% (v/v) to about 5% (v/v), the method comprising:
- obtaining a liquid medium containing platelets;
- suspending platelets in a suspension medium, the suspension medium comprising dimethyl sulfoxide (DMSO) in the amount of about 0.1% (v/v) to about 5% (v/v);
- and
- subjecting the suspended platelets in the suspension medium to a temperature suitable for forming the composition.
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A method of making a composition comprising:
- cryopreserved platelets;
- and
- a cryoprotectant,
- wherein the composition comprises dimethyl sulfoxide (DMSO), the method comprising:
- obtaining platelets contained in a liquid medium;
- suspending the platelets in a suspension medium comprising a cryoprotectant, the suspension medium comprising dimethyl sulfoxide (DMSO) in the amount of about 0.1% (v/v) to about 5% (v/v);
- and
- subjecting the suspended platelets to a temperature sufficient to form the composition.
A method of making a composition comprising:
- cryopreserved platelets;
- and
- a cryoprotectant comprising glycerol;
- wherein the composition comprises dimethyl sulfoxide (DMSO), the method comprising:
- obtaining a liquid medium containing platelets;
- suspending platelets in a suspension medium comprising a cryoprotectant, the suspension medium comprising dimethyl sulfoxide (DMSO) in the amount of about 0.1% (v/v) to about 5% (v/v);
- and
- subjecting the suspended platelets in the suspension medium to a temperature suitable for forming the composition.
Dependencies are inferred from the claim text.