Method for generating t cells progenitors
AssigneesAssistance Publique-Hopitaux de Paris, Fondation Imagine - Institut des Maladies Génétiques, Universite Paris Descartes, Institut National De La Sante Et De La Recherche Medicale (Inserm)
InventorsIsabelle André, Marina Cavazzana, Kuiying Ma, John Tchen
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Abstract
The invention relates to an in vitro method to generate T cell progenitors, comprising the step of culturing CD34+ cells in a medium containing TNF-alpha and/or an antagonist of the Aryl hydro-carbon/Dioxin receptor, in particular StemRegenin 1 (SR1), in presence of a Notch ligand and optionally a fibronectin fragment.
Claims21
-20. (canceled)
An in vitro method for generating T cells precursors, comprising the step of culturing CD34+ cells in a medium comprising TNF-alpha and/or an antagonist of the Aryl hydrocarbon/Dioxin receptor, in particular StemRegenin 1 (SR1), and in the presence of an immobilized Notch ligand.
A CD7+ T cell progenitors population wherein the proportion of cells that are CD34− and CD1a− or CD34− and CD5−, or CD34− and CD1a− and CD5− in this population is higher than 80%.
Dependencies are inferred from the claim text.