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US 2020/0046766 A1Published application
Cancer stem cell exosomes
AssigneeVbc Holdings LLC
InventorsKayvan NIAZI, Francesco CURCIO
Life of this patent
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Abstract
The invention includes methods for preparing inactivated exosomes isolated from cancer stem cells, as well as inactivated exosomes produced by the inventive method. The invention also provides methods for inhibiting the growth and/or progression of cancer, by administering inactivated exosomes to a subject.
Claims18
A process for preparing inactivated exosomes comprising the steps of:
- (a) isolating cancer stem cells (CSCs) from human cancer tissue,
- (b) contacting the isolated CSCs of (a), in a culture medium, with pro-inflammatory cytokines, at a concentration, and for a time period, sufficient to induce release of inflammation induced exosomes,
- (c) isolating inflammation induced exosomes from the culture medium of (b), and
- (d) inactivating the isolated exosomes of (c) while preserving the membrane structure of the isolated exosomes.
1 more element
A pharmaceutical composition comprising the exosomes of claim 11, and a physiologically acceptable carrier.
A process for preparing inactivated exosomes comprising the steps of:
- (a) isolating cancer stem cells (CSCs) from human cancer tissue,
- (b) contacting the isolated CSCs of (a), in a culture medium, with pro-inflammatory cytokines, at a concentration, and for a time period, sufficient to induce release of inflammation induced exosomes,
- (c) modifying the isolated CSCs of (a) or (b) to suppress transcription or translation of a gene or genes encoding a cancer promoting RNA or polypeptide, and
- (d) isolating inflammation induced exosomes from the culture medium of (b).
1 more element
Dependencies are inferred from the claim text.