System for extending dynamic range and contrast of a video under fluorescence imaging

A system for extending dynamic range and contrast of an image or a video under fluorescence imaging, the system comprising:

• a) a trigger generator (104) configured to generate Pulse Width Modulation (PWM) signals and transmit PWM signals to a light engine (107), wherein the trigger generator (104) provides trigger signals (104B) to a detector (103), wherein the trigger generator (104) provides a plurality of trigger signals (104A) to the light engine (107);
• b) the light engine (107) configured to provide different intensity of IR light of different wavelength, wherein the light engine includes one or more variable excitation light source (108) and one and more near infrared reflectance (NIR) light sources (109);
• c) a plurality of excitation filter (101) configured to transmit wavelengths of the light to the subject (110), wherein the excitation filter (101) receives at least one of high intensity fluorescence light (107A), low intensity fluorescence light (107B) and NIR reflectance light (107C) as inputs;
• d) a plurality of emission filter (102) configured to receive a light signal of wavelength from the subject;
• e) one or more detectors (103) configured to detect data from at least one of high intensity fluorescence data (105A), low intensity fluorescence data (105B) and NIR reflectance data (105C), wherein detector capture one or more image frame of received data, wherein the detector (103) is connected to the emission filter (102) to receive fluorescence light emitted by the subject (110);
• f) a scene processing unit (105) configured to read trigger signal (104A) from the detector (103), wherein the scene processing unit (105) includes high intensity fluorescence data (105A), low intensity fluorescence data (105B), wide dynamic range florescence data (105C), NIR reflectance data (105D) and enhanced vascular index data (105E);
• and
• g) a selective visualization unit (106) configured to visualize an image or video feed on a standard display, wherein the selective visualization unit (106) visualize five data streams available from scene processing unit (105), wherein the selective visualization unit (106) allows user to input least one of five data streams to visualize.