Methods of preserving the biological activity of ribonucleic acids
AssigneesSyngenta Participations AG, Devgen NV
InventorsPascale FELDMANN, Jeffrey David FOWLER, Nema Devi JHURRY, Isabelle MAILLET, Marta OMEDES PUJOL
Life of this patent
Estimating the term…
Abstract
The present invention provides a method of substantially retaining or otherwise preserving the biological activity of a dsRNA, present in a cell, to post-transcriptionally silence the expression of a gene in a target organism, comprising the step of adding to the cell composition a compound having the function of a protein—or amine—cross linking agent and/or an acid. The invention also comprises compositions comprising the cells comprising dsRNA, and protein cross linking agents and/or acids, as well as the use of said cross linking agents and/or acids in the method.
Claims15
A method of substantially retaining or otherwise preserving the biological activity of a dsRNA, present in a cell, to post-transcriptionally silence the expression of a gene in a target organism, comprising the step of adding to a composition comprising said cell a compound having the function of a protein—or amine—cross linking agent and/or an acid.
A composition of matter comprising a cell and a protein—or amine-cross linking agent and/or an acid, characterised in that the composition comprises soil, the cell comprises dsRNA, and the cross linking agent is glutaraldehyde and the acid is lactic acid or formic acid.
A cell composition comprising a protein—or amine—cross linking agent and/or an acid, added for the purpose of retaining the biological activity of a dsRNA heterologously expressed in the cell.
The use of a protein—or amine—cross linking agent and/or an acid to substantially stabilise or otherwise preserve the biological activity of a dsRNA present in a cell.
Dependencies are inferred from the claim text.