Compositions and Methods for Identifying and Selecting Brachytic Locus in Solanaceae
AssigneeUniversity of Florida Research Foundation, Inc.
InventorsTong Geon Lee, Samuel Forrest Hutton
Life of this patent
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Abstract
Molecular markers associated with Solanaceae brachytic locus, methods of their use, and compositions having one or more marker loci are provided. Methods comprising detecting at least one marker locus, detecting a haplotype, and/or detecting a marker profile are provided. Methods and compositions are described for breeding tomato plants containing quantitative trait loci that are associated with brachytic phenotype and monitoring introgression of brachytic locus. Methods and compositions are also described for modifying a brachytic locus using CRISPR/Cas.
Claims22
A method for producing a brachytic Solanaceae plant, comprising:
- (a) isolating one or more nucleic acids from one or more Solanaceae plants;
- (b) genotyping said one or more nucleic acids for the presence of a brachytic marker, wherein the brachytic marker comprises a detectable genetic marker linked, closely linked, tightly linked, or extremely tightly linked to any of SEQ ID NOs:
- 1-8 and 48 or an ortholog thereof, or a fragment of any of SEQ ID NOs:
- 1-8 and 48 or an ortholog thereof;
- (c) selecting a first Solanaceae plant on the basis of the presence of the brachytic marker genotyped in (b);
- (d) crossing the first Solanaceae plant of (c) with a second Solanaceae plant not having the brachytic marker;
- (e) collecting seed from the cross of (d);
- and
- (f) growing a progeny Solanaceae plant from the seed of (e), wherein said progeny Solanaceae plant comprises in its genome the brachytic marker.
A method of introgressing a brachytic (br) allele into a Solanaceae plant, comprising:
- (a) crossing at least one donor Solanaceae plant known to have at least one br allele with at least one recipient Solanaceae plant in order to form a segregating population;
- and
- (b) screening the segregating population with one or more brachytic markers to determine if one or more Solanaceae plants from said segregating population contains the one or more brachytic markers, wherein the one or more brachytic makers comprise one or more detectable genetic markers linked, closely linked, tightly linked, or extremely tightly linked to any of SEQ ID NOs:
- 1-8 and 48 or an ortholog thereof, or a fragment of any of SEQ ID NOs:
- 1-8 and 48 or an ortholog thereof.
A method for selecting a plant having or expected to have short internode length and/or early flowering, comprising identifying in the plant one or more brachytic markers known to be linked a br allele wherein the one or more brachytic markers comprise detectable genetic markers linked, closely linked, tightly linked, or extremely tightly linked to any of SEQ ID NOs:
- 1-8 and 48 or an ortholog thereof, or a fragment of any of SEQ ID NOs:
- 1-8 and 48 or an ortholog thereof.
A genetically modified Solanaceae plant wherein a brachytic locus has been genetically modified through the use of a CRISPR/Cas system.
A method of genetically modifying a Solanaceae plant, the method comprising:
- introducing a Cas protein or a nucleic acid encoding the Cas protein and a guide RNA or a nucleic acid encoding the guide RNA into a plant cell, wherein the guide RNA and Cas protein form a complex that targets the brachytic locus.
Dependencies are inferred from the claim text.