STABILIZED TWO-PART HEMATOXYLIN SOLUTION UTILIZING pH ADJUSTMENT
AssigneeVentana Medical Systems, Inc.
InventorsEdward E. Durrant, Christopher A. Koerber
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Abstract
The present disclosure provides stabilized hematoxylin formulations having a pH of less than 2.4. The present disclosure also provides methods of using such stabilized hematoxylin formulations to stain biological samples.
Claims20
A stabilized hematoxylin formulation comprising hematoxylin dye and an acid in an amount ranging from about 0.2% to about 4% by total volume of the hematoxylin solution, and wherein the hematoxylin solution has a pH of less than 2.4, wherein the acid is selected from the group consist of hydrochloric acid, sulfuric acid, perchloric acid and nitric acid.
A kit comprising a first component and a second component,
- the first component comprising a stabilized hematoxylin formulation comprising a hematoxylin dye and an acid in an amount ranging from about 0.1% to about 10% by total volume of the stabilized hematoxylin formulation, and wherein the stabilized hematoxylin formulation has a pH of less than 2.4;
- and
- the second component comprising a strong base or a buffer, the second component provided in an amount relative to the first component such that when the first and second components are mixed, the pH of the hematoxylin solution increases to between about 2.4 to about 2.6.
Dependencies are inferred from the claim text.