Decellularized Tissue as a Microcarrier for Cell Culture and Expansion

An injectable or implantable tissue regeneration product comprising:

• a microcarrier for cell culture and expansion, wherein the microcarrier includes decellularized mammalian tissue and has an average particle size ranging from about 10 micrometers to about 600 micrometers;
• mammalian cells harvested from mammalian tissue of a same type as the decellularized mammalian tissue;
• one or more growth factors;
• and
• a gel precursor or a pre-formed scaffold.

A method of forming a decellularized mammalian tissue microcarrier for cell culture and expansion, the method comprising:

• obtaining a mammalian tissue sample;
• decellularizing the mammalian tissue sample;
• and
• micronizing the mammalian tissue sample to form the decellularized mammalian tissue microcarrier, wherein the decellularized mammalian tissue microcarrier has an average particle size ranging from about 10 micrometers to about 600 micrometers.

A method of treating a mammalian tissue defect, the method comprising:

• obtaining a decellularized mammalian tissue microcarrier, wherein the decellularized mammalian tissue microcarrier has an average particle size ranging from about 10 micrometers to about 600 micrometers;
• expanding cells harvested from a mammalian tissue sample of a same type as the decellularized mammalian tissue microcarrier on the decellularized mammalian tissue microcarrier inside a cell culture vessel;
• and
• injecting or implanting the decellularized mammalian tissue microcarrier with the expanded cells attached thereto into the mammalian tissue defect.