Novel System for the Biocontrol of Pathogens in Aquaculture and Other Animal Systems
AssigneePebble Labs USA Inc.
InventorsRichard T. Sayre, Tatiana Vinogradova-Shah
Life of this patent
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Abstract
The inventive technology relates to novel paratransgenic strategies for the biocontrol of pathogens in animal systems using interfering RNA molecules expressed in genetically modified bacteria that may be configured to colonize a target host. In one preferred embodiment, the invention includes novel paratransgenic strategies for the biocontrol of pathogens in aquatic organisms raised in aquaculture environments.
Claims30
1-7. (canceled)
A genetically modified probiotic bacteria configured for the biocontrol of white spot syndrome virus in shrimp comprising:
- a genetically modified probiotic enteric bacteria that expresses at least one heterologous inhibitory polynucleotide selected from the group consisting of:
- SEQ ID NO. 1, SEQ ID NO. 2, and/or SEQ ID NO. 3, wherein said at least one heterologous inhibitory polynucleotide is double stranded RNA polynucleotide molecule (dsRNA) that targets an essential gene in a white spot syndrome virus, and wherein said genetically modified probiotic enteric bacteria is configured to be probiotic to shrimp.
(canceled)
A genetically modified probiotic bacteria configured for the biocontrol of white spot syndrome virus in shrimp comprising:
- a genetically modified RNaseIII deficient probiotic bacteria that expresses at least one heterologous inhibitory polynucleotide selected from the group consisting of SEQ ID NO. 2, wherein said at least one heterologous inhibitory polynucleotide is double stranded RNA polynucleotide molecule (dsRNA) that targets the vp28 gene in a white spot syndrome virus identified as SEQ ID NO. 8, and wherein administration of a therapeutically effective amount of said genetically modified RNaseIII deficient probiotic bacteria to a shrimp result in a reduction in mortality due to white spot syndrome virus.
(canceled)
An aquatic organism ingestible compound for the biocontrol of white spot syndrome comprising:
- a treated feed for aquatic organisms having a genetically modified probiotic bacteria that expresses at least one heterologous inhibitory polynucleotide selected from the group consisting of:
- SEQ ID NO. 1, SEQ ID NO. 2, and/or SEQ ID NO. 3, wherein said at least one heterologous inhibitory polynucleotide is double stranded RNA polynucleotide molecule (dsRNA) that targets an essential gene in a white spot syndrome virus.
A shrimp ingestible compound for the biocontrol of white spot syndrome comprising:
- a treated feed for shrimp having a genetically modified probiotic enteric bacteria that expresses at least one heterologous inhibitory polynucleotide selected from the group consisting of SEQ ID NO. 1, SEQ ID NO. 2, and/or SEQ ID NO. 3, wherein said at least one heterologous inhibitory polynucleotide is double stranded RNA polynucleotide molecule (dsRNA) that targets an essential gene in a white spot syndrome virus.
36-73. (canceled)
Dependencies are inferred from the claim text.