Production of xylitol from glucose by a recombinant strain
AssigneeRoquette Freres
InventorsAstrid SCHAEFER, Melanie DIEFENBACHER, Yiming CHANG, Sumire HONDA MALCA, Markus SCHWAB, Sophie DEFRETIN, Tania GERARD, Arnaud HEYSEN, Friederike THOR
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Abstract
The present invention relates to a recombinant microbial host for the production of xylitol, the recombinant microbial host containing a nucleic acid sequence encoding a NAD+-specific D-arabitol 4-oxidoreductase (EC 1.1.1.11) using D-arabitol as substrate and producing D-xylulose as product, and a nucleic acid sequence encoding a NADPH-specific xylitol dehydrogenase using D-xylulose as substrate and producing xylitol as product.
Claims20
A method for producing xylitol, the method comprising:
- culturing in a culture medium a recombinant host cell comprising:
- a heterologous nucleic acid sequence encoding a NAD+-specific D-arabitol 4-oxidoreductase (EC 1.1.1.11) using D-arabitol as a substrate and producing D-xylulose as a product;
- and
- a heterologous nucleic acid sequence encoding a NADPH-specific xylitol dehydrogenase using D-xylulose as a substrate and producing xylitol as a product;
- and
- recovering the produced xylitol,
The method according to claim 5, wherein the host cell produces D-arabitol from D-glucose under a high osmotic pressure medium.
Dependencies are inferred from the claim text.