Compositions and methods for long-term in vitro culture of the syphilis spirochete
AssigneeBoard of Regents, The University of Texas System
InventorsSteven J. NORRIS, Diane G. EDMONSON
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Abstract
Presented herein are compositions and methods for the long-term in vitro culturing of Treponema species such as T. pallidum. Culture media and systems for Treponema culture are also provided.
Claims27
A culture system comprising a co-culture of a population of Treponema species organisms and a population of mammalian epithelial cells, wherein said co-culture is present in microaerobic conditions and in the presence of a medium comprising excess nutrients and wherein the said Treponema species organisms are in direct contact with the mammalian epithelial cells.
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A culture medium comprising CMRL 1066, sodium pyruvate, Resazurin, a buffering agent, fetal bovine serum, at least one amino acid, at least one sugar or sugar alcohol and at least one reducing agent.
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Dependencies are inferred from the claim text.