Method for determining affinity of a biomolecule

Abstract

Disclosed is a method for determining affinity of a biomolecule, including the steps of: determining a theoretical saturation response value (Rmax) based on known properties of the biomolecule or a similar biomolecule; providing a sensor surface having the biomolecule immobilized thereon as a ligand; contacting the sensor surface with a plurality of samples containing different concentrations of an analyte that is able to bind to the ligand; registering an equilibrium response (R) from binding of the analyte to binding sites of the ligand for each of the plurality of samples, said sensor response giving equilibrium response values for each sample; creating an equilibrium response sequence comprising the determined equilibrium response values; determining a plurality of values for a dissociation constant for the plurality of concentrations based on said equilibrium response sequence; and determining a theoretical dissociation constant at zero concentration based on the plurality of values for the dissociation constant.

Dependencies are inferred from the claim text.