Sensor for nadp (h) and development of alcohol dehydrogenases

A method for isolating genes which code for NADP(H)-dependent enzymes, comprising the method steps:

• (I) providing an NADP(H) nanosensor comprising
• i) a nucleic acid sequence to which a regulator is capable of binding, wherein the oxidation state of the regulator depends on the NADP(H) availability;
• ii) a promoter sequence following the nucleic acid sequence i), to which an RNA polymerase is capable of binding, wherein the affinity of the RNA polymerase for the promoter sequence is influenced by the oxidation state of the regulator;
• iii) a nucleic acid sequence which is under the control of the promoter sequence ii) and which codes for an autofluorescent protein;
• (II) introducing the NADP(H) nanosensor into a cell;
• (III) introducing a gene which may code for an NADP(H)-dependent enzyme into individual cells of a cell suspension of the cells obtained in method step (II);
• (IV) incubating the cells with a substrate for the NADP(H)-dependent enzyme;
• (V) identifying individual cells in the cell suspension with an increased activity of NADP(H)-dependent enzymes by detection of the intracellular fluorescence activity;
• (VI) separating off the identified cells from the cell suspension;
• (VII) isolating the genes coding for an NADP(H)-dependent enzyme in the identified cells.