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US 2020/0025782 A1Published application
Automated cell processing systems and methods
AssigneeGenesis Technologies Limited
InventorJan-Eric Ahlfors
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AssigneeGenesis Technologies Limited
InventorJan-Eric Ahlfors
Estimating the term…
Systems and methods for automated cell processing of biological samples, such as cells for use in cell therapy and regenerative medicine. Systems for automated processing of batches derived from biological samples comprise: a closed and sterile enclosure; a plurality of reagent containers; at least one reagent dispenser; a quality control module for analyzing at least one characteristic of a batch; a harvesting module; a robotic module; and a control unit (CU) communicatively coupled to the at least one reagent dispenser, the quality control module, the harvesting module and the robotic module for controlling the automatic processing of batches. The automatic processing may be executable without handling by a human operator. The system may be configured to automatically process the plurality of batches without cross-contamination between batches, e.g., under GMP conditions.
A system for automated processing of batches, the batches being derived from biological samples, comprising:
14A. The system of any one of claims 1 to 13, further comprising:
20A. The system of claim 20, wherein the liquid is cell culture media.
A system for automated processing of a plurality of batches, the batches being derived from biological samples, comprising:
49A. The system of any one of claims 34 to 48, further comprising:
55A. The system of claim 55, wherein the liquid is cell culture media.
An automated method for processing a batch in a closed and sterile enclosure, the batch being derived from a biological sample inserted into the enclosure, the automated method comprising:
An automated method for processing a batch in a closed and sterile enclosure, the batch being derived from a biological sample inserted into the enclosure, the automated method comprising:
A method for automated sterilization of an enclosure for processing of batches comprising:
A robotic aspirator comprising:
A method of aspirating using a robotic arm having a fluid flow channel and a plurality of prongs configured to selectively hold a disposable tip in fluid connection with the fluid flow channel, the method comprising:
The system of any one of the preceding claims, comprising an automated liquid fill station housed inside the enclosure and configured for direct filling of liquid from a supply container disposed inside or outside the enclosure into a cell processing container disposed inside the enclosure, optionally wherein the liquid is media, optionally wherein the liquid fill station is configured to dispense more than 5 mL of liquid at a time.
The method of any one of the preceding claims, comprising a step of direct filling a liquid from a supply container disposed inside or outside the enclosure into a cell processing container disposed inside the enclosure, optionally wherein the liquid is media, optionally wherein more than 5 mL of liquid is dispensed at a time.
The system of any one of the preceding claims, comprising robotic means for tilting a cell processing container, for delivery of cells or media thereto and/or removal of cells or media therefrom.
The system of any one of the preceding claims, comprising robotic means for capping and decapping screwcap lids of containers.
The system of any one of the preceding claims, comprising automated means for purifying cell mixtures or obtaining a cell pellet.
The system of any one of the preceding claims, comprising means for incubating cells.
The system of any one of the preceding claims, comprising automated means for determining cell number or cell confluency in a sample.
The system of any one of the preceding claims, comprising a vertical waste chute for discarding solid waste configured so that the waste does not hit the edges of the chute, and placed under sufficient negative pressure such that particles from the chute do not enter the enclosure.
The system of any one of the preceding claims, comprising sterilization means for sterilizing the enclosure.
The system of any one of the preceding claims, comprising robotic means for freezing of batches and/or means for manipulation of samples or reagents at subzero temperatures.
The system of any one of the preceding claims, wherein the system further comprises a module for quality assurance.
A method for automated processing of cells, the method comprising the steps of:
A biological sample prepared using the automated system or method of any one of the preceding claims.
A population of in vitro human multipotent, unipotent, or somatic cells derived from the reprogramming of a somatic cell, a progenitor cell or a stem cell of a different type using the automated system or method of any one of the preceding claims.
An automated process for reprogramming a cell of a first type to a desired cell of a different type that is multipotent or unipotent, the cell of a first type being a somatic cell, a stem cell, or a progenitor cell, the automated process executable by the system of any one of the preceding claims, the automated process comprising:
Dependencies are inferred from the claim text.