Kit, method, and reagent for measuring measurement target substance

A method for measuring a measurement target substance, the method comprising:

• a step (i) of mixing (a) first particle having a label and modified with a first binding substance capable of specifically binding to a measurement target substance, (b) second particle having no label and modified with a second binding substance incapable of specifically binding to the measurement target substance, and (c) test sample solution containing the measurement target substance to obtain a liquid mixture;
• a step (ii) of applying the liquid mixture obtained in the step (i) on a substrate;
• a step (iii) of capturing the measurement target substance or the first binding substance in a reaction site on the substrate which has a third binding substance capable of specifically binding to the measurement target substance or a substance capable of binding to the first binding substance;
• and
• a step (iv) of detecting the measurement target substance or the first binding substance captured on the reaction site,
• wherein the first particle having a label is a luminescent labeled particle containing at least one kind of compound represented by Formula (1) and a particle,
• in the formula, R11 to R15 each independently represent a hydrogen atom, a halogen atom, an alkyl group, an aryl group, a heterocyclic group, an ethenyl group, an ethynyl group, an amino group, an acyl group, an alkoxy group, an aryloxy group, an alkylthio group, or an arylthio group, each of which may have a substituent, and at least three of R11, . . . , or R15 represent atoms or groups other than hydrogen atoms;
• X1 and X2 each independently represent a halogen atom, an alkyl group, an aryl group, a heterocyclic group, a hydroxy group, an alkoxy group, an aryloxy group, an alkylthio group, an arylthio group, an ethenyl group, or an ethynyl group, each of which may have a substituent, and X1 and X2 may be linked to each other to form a ring;
• Ar1 and Ar2 each independently represent an aryl group or a heterocyclic group, each of which may have a substituent;
• and L1 and L2 each independently represent any one of Formulae (L-1) to (L-4),
• in the formulae, R111 to R116 each independently represent a hydrogen atom, a halogen atom, an alkyl group, an aryl group, a heterocyclic group, an ethenyl group, an ethynyl group, an amino group, an acyl group, an alkoxy group, an aryloxy group, an alkylthio group, or an arylthio group, each of which may have a substituent;
• and A represents —O—, —S—, or —NH—.

A reagent for measuring a measurement target substance, the reagent comprising:

• (a) first particle having a label and modified with a first binding substance capable of specifically binding to a measurement target substance;
• and
• (b) second particle having no label and modified with a second binding substance incapable of specifically binding to the measurement target substance,
• wherein the first particle having a label is a luminescent labeled particle containing at least one kind of compound represented by Formula (1) and a particle,
• in the formula, R11 to R15 each independently represent a hydrogen atom, a halogen atom, an alkyl group, an aryl group, a heterocyclic group, an ethenyl group, an ethynyl group, an amino group, an acyl group, an alkoxy group, an aryloxy group, an alkylthio group, or an arylthio group, each of which may have a substituent, and at least three of R11, . . . , or R15 represent atoms or groups other than hydrogen atoms;
• X1 and X2 each independently represent a halogen atom, an alkyl group, an aryl group, a heterocyclic group, a hydroxy group, an alkoxy group, an aryloxy group, an alkylthio group, an arylthio group, an ethenyl group, or an ethynyl group, each of which may have a substituent, and X1 and X2 may be linked to each other to form a ring;
• Ar1 and Ar2 each independently represent an aryl group or a heterocyclic group, each of which may have a substituent;
• and L1 and L2 each independently represent any one of Formulae (L-1) to (L-4),
• in the formulae, R111 to R116 each independently represent a hydrogen atom, a halogen atom, an alkyl group, an aryl group, a heterocyclic group, an ethenyl group, an ethynyl group, an amino group, an acyl group, an alkoxy group, an aryloxy group, an alkylthio group, or an arylthio group, each of which may have a substituent;
• and A represents —O—, —S—, or —NH—.