Non-target site resistance
AssigneeUniversity of Newcastle Upon Tyne
InventorsRobert Edwards, Catherine Tetard-Jones, Nawaporn Onkokesung, Melissa Catherine Brazier-Hicks, Alina Viviana Goldberg Cavalleri
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Abstract
The present invention provides novel biomarkers for non-target-site resistance (NTSR) in wild grass, with corresponding methods of identifying NTSR. Corresponding kits and assay devices are also provided.
Claims19
A method for detecting non-target site herbicide resistance (NTSR) in wild grass, comprising using at least one of:
- i. GSTU2;
- ii. D-3-phosphoglycerate dehydrogenase 1;
- iii. 12-oxophytodienoate reductase 1;
- iv. GSTF2;
- v. NADPH:quinone oxidoreductase 1;
- vi. NAD-dependent epimerase/dehydratase;
- or
- vii. stem-specific protein TSJT1;
- wherein the NTSR is based upon metabolic based resistance.
A method of identifying non-target-site herbicide resistance in wild grass, the method comprising:
- i) determining the level of at least one biomarker selected from a group consisting of:
- GSTU2, D-3-phosphoglycerate dehydrogenase 1, 12-oxophytodienoate reductase 1, GSTF2, NADPH:
- quinone oxidoreductase 1, NAD-dependent epimerase/dehydratase and stem-specific protein TSJT1 in a test sample of the wild grass;
- and
- ii) comparing the level of the at least one biomarker in the test sample with the level of the at least one biomarker in a control sample or with a predetermined reference level for the at least one biomarker;
- wherein an increased level of the at least one biomarker in the test sample compared to the control sample or compared to the predetermined reference level is indicative of non-target-site herbicide resistance based upon metabolic based resistance.
A kit for identifying non-target-site herbicide resistance (NTSR) in wild grass, wherein the NTSR is based upon metabolic based resistance, the kit comprising:
- a detectably labelled agent that specifically binds to a biomarker selected from a group consisting of:
- GSTU2, D-3-phosphoglycerate dehydrogenase 1, 12-oxophytodienoate reductase 1, GSTF2, NADPH:quinone oxidoreductase 1, NAD-dependent epimerase/dehydratase and stem-specific protein TSJT1.
An assay device for identifying non-target-site herbicide resistance (NTSR) of wild grass, wherein the NTSR is based upon metabolic based resistance, the device comprising:
- a surface with a detectably labelled agent located thereon, wherein the detectably labelled agent specifically binds to a biomarker selected from a group consisting of:
- GSTU2, D-3-phosphoglycerate dehydrogenase 1, 12-oxophytodienoate reductase 1, GSTF2, NADPH:
- quinone oxidoreductase 1, NAD-dependent epimerase/dehydratase and stem-specific protein TSJT1.
Dependencies are inferred from the claim text.