Methods for determination of polar pesticides by chromatography

A kit comprising:

• (i) a reversed-phase chromatography system comprising
• (a) a reversed phase column having a stationary phase material contained inside the column, the stationary phase material comprising a phenyl functional group, and
• (b) a detector;
• and
• (ii) instructions for
• (a) obtaining a sample comprising at least one of glyphosate or AMPA in a sample matrix;
• (b) introducing the sample to the reversed-phase chromatography system;
• (c) flowing the sample with a mobile phase eluent through the reversed-phase column, wherein the at least one of glyphosate or AMPA is substantially resolved and retained, the mobile phase eluent having a pH between about 1.5 to about 2.5;
• and
• (d) detecting the at least one of glyphosate or AMPA using a detector.

A kit comprising:

• (i) a reversed-phase chromatography column having a stationary phase material contained inside the column, the stationary phase material comprising a phenyl functional group;
• (ii) instructions contained in a computer readable medium for operating a reversed-phased chromatography system, wherein the instructions automate
• inserting a sample into a mobile phase eluent;
• flowing the sample with the mobile phase eluent through the reversed-phase chromatography column;
• and
• detecting components of the sample;
• and
• (iii) a vial containing the mobile phase eluent acidified to a pH of about 2.