Analytical Laboratory Testing Procedures
AssigneePrompt Praxis Laboratories LLC
InventorsLisa McChesney, Cecilia Newcomb
Life of this patent
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Abstract
A test method developed with the goals of rapid turnaround, short run times (target under 5 minutes), small volumes for dilution (target 10 mL), fewest number of mobile phases, rapid transition between analyses, minimized opportunity for preparation errors, leverage Autoblend® function of equipment, easily maintain on-hand solution inventory, and avoiding ion-pairing agents and other additives. The test method development strategy begins with vendor application notes, USP, and literature references. The strategy targets families of chemically similar compounds, whenever possible, and leverages new column technologies. Finally, the strategy standardizes validation and sample preparation procedures.
Claims9
A liquid chromatography testing method for reducing laboratory waste and testing times, the method comprising the steps of:
- accepting a plurality of samples to be tested on a liquid chromatograph;
- preparing at least one analyte for each of the plurality of samples to create a plurality of test analytes, wherein the step of preparing at least one analyte comprises the steps of:
- limiting a volumetric size of laboratory glassware used to no more than 10.0 mL;
- limiting transfer of liquids to be no more than 100 microliters;
- utilizing ultra-high-performance liquid chromatography;
- selecting autosampler vials to allow analysis of as little as 300 microliters of sample;
- grouping analytes by families to allow a single chromatographic column to be used for all analytes in a family;
- running a combination standard for multiple chemicals on the liquid chromatograph;
- and
- creating a subset of the plurality of test analytes based on those containing at least one of the multiple chemicals in the combination standard.
An analytical testing procedure comprising the steps of:
- receiving a plurality of samples to be analyzed;
- grouping the plurality of samples by chemical family;
- preparing test samples for each of the plurality of samples comprising the step of transferring no more than 100 microliters of each sample to an autosampler vial to create a plurality of test samples;
- analyzing each of the plurality of test samples by family groupings, comprising the steps of:
- running test samples through an ultra high-performance liquid chromatograph;
- capturing data from the ultra high-performance liquid chromatograph in an electronic notebook;
- reviewing captured data in electronic notebook for anomalies;
- reporting results of testing.
Dependencies are inferred from the claim text.