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US 2020/0025657 A1Published application
Methods and devices for high throughput purification
AssigneesUniversity of Maryland, Baltimore, The Trustees of Princeton University
InventorsJoseph D'SILVA, Robert H. AUSTIN, Curt CIVIN, James C. STURM
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Abstract
Described herein are devices and methods for high throughput purification of particles. In some cases, methods and devices described herein can be used to remove erythrocytes and purify leukocytes and raise the quality of umbilical cord blood and other transplant grafts, thereby significantly improving patient outcomes.
Claims13
A method of purifying first particles of at least a predetermined size, the method comprising:
- (a) applying at least 100 mL of a sample comprising the first particles of at least the predetermined size to a deterministic lateral displacement microfluidic array, wherein the sample comprises blood, and further comprises:
- (i) a calcium chelator at a concentration sufficient to inhibit clot formation when the sample is applied to the array;
- and/or
- (ii) a direct thrombin inhibitor at a concentration sufficient to inhibit clot formation when the sample is applied to the array;
- and
- (b) flowing the at least 100 mL of the sample through the array at a rate of at least 1 mL/min, wherein the array comprises an array of obstacles arranged in rows, wherein each subsequent row of obstacles of the array of obstacles is shifted laterally with respect to a previous row, wherein the obstacles of the array of obstacles differentially deflect the first particles of at least the first predetermined size to a first outlet and second particles in the sample of less than the predetermined size to a second outlet, thereby purifying the first particles of at least the predetermined size.
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Dependencies are inferred from the claim text.