Nanopipette Analysis of Polymers
OwnerKalim Mirinventor
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Abstract
The disclosure relates to devices and instruments for detecting and individually analyzing biomolecules, biomolecular complexes and biomolecules with ligands attached thereon.
Claims10
1-42. (canceled)
A method of analyzing one or more macromolecules, the method comprising:
- i. attaching one or more macromolecules to a fixed location on a solid phase surface;
- ii. providing a first electrode in fluidic communication with said one or more macromolecules through an ionic aqueous buffer;
- iii. providing a nanopipette containing a second electrode;
- iv. applying a potential difference between the first and second electrodes to induce the flow of ions;
- v. bringing the nanopipette in sufficient proximity to a macromolecule such that the macromolecule enters into the nanopipette;
- and
- vi. measuring an ion flow,
- wherein the nanopipette is one of an array of nanopipettes configured for entry of a macromolecule.
The method according to 43 where the array of nanopipettes comprise pulled glass capillaries or monolithic, nano-fabricated or micro-fabricated structures.
The method according to 43 where the array of nanopipettes are configured to obtain measurements of a number of macromolecules in parallel.
The method according to 43 where the array of nanopipettes are configured to obtain measurements of a number of macromolecules in a cell in parallel.
The method according to 43 where the array of nanopipettes are configured to obtain measurements of macromolecules in a number of cells in parallel.
The method according to 43, wherein each nanopipette in the array is located precisely at a location of individual wells in a microwell array
The method according to 43, wherein the array comprises individual reference nanopipettes with no macromolecules as a control.
The method according to 43, wherein the nanopipettes comprise nanopores.
The method according to 50, wherein the nanopores are embedded within a surface and the surface containing the nanopores is translated in the z direction with respect to the surface to which the macromolecules are attached.
Dependencies are inferred from the claim text.