Bioluminescent assays utilising secreted luciferases
AssigneeGene Stream Pty Ltd.
InventorsMarco Peter Leu, John Michael Daly
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Abstract
Disclosed herein are methods for determining the amount or activity of one or more luciferases and methods for measuring the luminescent signal generated by one or more luciferases in a sample, the methods comprising incubating the sample with a reactive substrate(s) of the luciferase(s) to be analysed and a reducing agent to inactivate a first luciferase, wherein the first luciferase, in its native form, is a secreted luciferase.
Claims16
1-68. (canceled)
A method for inactivating a luciferase that, in its native form, is a secreted luciferase, the method comprising contacting a sample containing the luciferase with a reducing agent.
A method for increasing the rate of decay of the luminescent signal generated by a luciferase that, in its native form, is a secreted luciferase, the method comprising contacting a sample containing the luciferase with a reducing agent.
A kit for use in assaying the amount and/or activity of one or more luciferases, the kit comprising at least one reagent composition wherein at least one of the at least one reagent composition comprises a reducing agent and coelenterazine or a derivative thereof, and does not comprise ethylenediaminetetraacetic acid (EDTA).
Dependencies are inferred from the claim text.