Improved glycerol free ethanol production
AssigneeDsm IP Assets B.V.
InventorsPaulus Petrus DE WAAL, Jozef Petrus Johannes SCHMITZ, Hans Marinus Charles Johannes DE BRUIJN
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Abstract
The invention relates to a recombinant cell, preferably a yeast cell comprising one or more genes coding for an enzyme having glycerol dehydrogenase activity, one or more genes coding dihydroxyacetone kinase (E.C. 2.7.1.28 and/or E.C. 2.7.1.29); one or more genes coding for an enzyme in an acetyl-CoA-production pathway and one or more genes coding for an enzyme having at least NAD+ dependent acetylating acetaldehyde dehydrogenase activity (EC 1.2.1.10 or EC 1.1.1.2), and optionally one or more genes coding for a glycerol transporter. This cell can be used for the production of ethanol and advantageously produces little or no glycerol.
Claims22
A recombinant cell, optionally a yeast cell, said recombinant cell comprising:
- one or more genes coding for an enzyme having glycerol dehydrogenase activity;
- one or more genes coding dihydroxyacetone kinase (E.C. 2.7.1.28 and/or E.C. 2.7.1.29);
- one or more genes coding for an enzyme in an acetyl-CoA-production pathway;
- and
- one or more genes coding for an enzyme having at least NAD+ dependent acetylating acetaldehyde dehydrogenase activity (EC 1.2.1.10 or EC 1.1.1.2);
- and optionally
- one or more genes coding for a glycerol transporter.
Dependencies are inferred from the claim text.