Efficient cell free production of papillomavirus gene transfer vectors
AssigneeThe USA, as represeented by the Secretary, Dept. of Health and Human Services
InventorsJohn T. SCHILLER, Carla V. CORREIA CERQUEIRA,, Patricia M. DAY, Douglas R. LOWY, David J. FITZGERALD
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Abstract
Methods of preparing papillomavirus nucleic acid transfer vectors, in-disassembled cluding by disassembly/reassembly of papillomavirus L1 and L2 virus-like particles, in a defined, cell-free high-efficiency production protocol. These methods may be used to efficiently encapsidate desired moieties, e.g., toxic or therapeutic nucleic acids such as DNA and RNA, and the resultant pseudovirus particles may be used as in vivo delivery vehicles.
Claims21
1-13. (canceled)
A method of producing a papillomavirus pseudovirus comprising a therapeutic nucleic acid molecule, the method comprising:
- a. mixing a virus like particle (VLP) comprising papillomavirus L1 and L2 proteins, with the therapeutic nucleic acid molecule, wherein the mixture lacks cellular factors;
- and,
- b. incubating the mixture under conditions such that the VLP encapsidates the therapeutic nucleic acid molecule, thereby producing a papillomavirus pseudovirus comprising the therapeutic nucleic acid molecule.
The method of claim 1, wherein the papillomavirus pseudovirus comprising the therapeutic nucleic acid molecule has an infectivity to particle ratio of at least 1×108 i.u./mg L1 protein.
The method of claim 1, wherein the mixture comprises less than 600 mM NaCl.
The method of claim 1, wherein the pH of the mixture is in the range of from 5.2 to less than 8.2.
The method of claim 1, wherein the step of mixing comprise contacting the VLP with at least 50 ng of the therapeutic nucleic acid molecule.
The method of claim 1, wherein the L1 and L2 proteins are from a HPV type selected from the group consisting of α4, α5, α7, α8, α9, α10, β1 and β1.
The method of claim 1, wherein prior to the step of incubating the mixture to encapsidate the therapeutic nucleic acid molecule, the VLP is disassembled.
A papillomavirus pseudovirus that comprises a therapeutic nucleic acid molecule, produced according a method comprising:
- a. mixing a virus like particle (VLP) comprising papillomavirus L1 and L2 proteins, with the therapeutic nucleic acid molecule, wherein the mixture lacks cellular factors;
- and,
- b. incubating the mixture under conditions such that the VLP encapsidates the therapeutic nucleic acid molecule, thereby producing a papillomavirus pseudovirus comprising a therapeutic nucleic acid molecule.
Dependencies are inferred from the claim text.