Systems and methods for visualization of single-cell resolution characteristics

A method for visualizing a pattern in a discrete attribute value dataset, the method comprising:

• obtaining a dataset that comprises:
• (i) a plurality of compressed contiguous blocks of data, wherein each contiguous block of data represents a different single-cell characteristic, in a plurality of single-cell characteristics, for each cell in a plurality of cells across each bin in a plurality of bins, and wherein each respective bin in the plurality of bins maps to a different portion of a reference genome, and
• (ii) a plurality of compressed downsampled contiguous blocks of data, each compressed downsampled contiguous block of data representing a different contiguous block in the plurality of contiguous blocks in downsampled form across the plurality of bins, and wherein
• the plurality of cells is clustered based upon the different single-cell characteristic, in a first contiguous block in the plurality of blocks, across each respective bin in the plurality of bins thereby forming a tree structure that includes a root node, a plurality of intermediate nodes, and a plurality of cells, and
• the respective cells in the plurality of cells constitute terminal nodes in the tree structure and each respective intermediate node has a corresponding plurality of daughter nodes, each daughter node being an intermediate node or a cell;
• displaying a subset of the tree on a first portion of the screen, wherein the subset includes the root node and a plurality of leaves, and wherein each leaf in the plurality of leaves represents an intermediate node in the plurality of intermediate nodes or a cell in the plurality of cells;
• displaying a heat map of the different single-cell characteristic associated with the downsampled contiguous block that represents the first contiguous block on a second portion of the screen, the heat map including a respective segment for each leaf in the plurality of leaves, across all or a portion of the plurality of bins, the respective segment including an average of the different single-cell characteristic across all the daughter nodes of a leaf when the leaf represents an intermediate node;
• and
• independently plotting two or more single-cell characteristics in the plurality of single-cell characteristics for the root node of the tree on a third portion of the screen using the corresponding downsampled contiguous blocks representing the two or more single-cell characteristics, across all or the portion of the plurality of bins.

A non-transitory computer readable storage medium, wherein the non-transitory computer readable storage medium stores instructions, which when executed by a computer system, cause the computer system to perform a method for visualizing a pattern, the method comprising:

• obtaining a dataset that comprises:
• (i) a plurality of compressed contiguous blocks of data, wherein each contiguous block of data represents a different single-cell characteristic, in a plurality of single-cell characteristics, for each cell in a plurality of cells across each bin in a plurality of bins, and wherein each respective bin in the plurality of bins maps to a different portion of a reference genome, and
• (ii) a plurality of compressed downsampled contiguous blocks of data, each compressed downsampled contiguous block of data representing a different contiguous block in the plurality of contiguous blocks in downsampled form across the plurality of bins, and wherein
• the plurality of cells is clustered based upon the different single-cell characteristic, in a first contiguous block in the plurality of blocks, across each respective bin in the plurality of bins thereby forming a tree structure that includes a root node, a plurality of intermediate nodes, and a plurality of cells, and
• the respective cells in the plurality of cells constitute terminal nodes in the tree structure and each respective intermediate node has a corresponding plurality of daughter nodes, each daughter node being an intermediate node or a cell;
• displaying a subset of the tree on a first portion of the screen, wherein the subset includes the root node and a plurality of leaves, and wherein each leaf in the plurality of leaves represents an intermediate node in the plurality of intermediate nodes or a cell in the plurality of cells;
• displaying a heat map of the different single-cell characteristic associated with the downsampled contiguous block that represents the first contiguous block on a second portion of the screen, the heat map including a respective segment for each leaf in the plurality of leaves, across all or a portion of the plurality of bins, the respective segment including an average of the different single-cell characteristic across all the daughter nodes of a leaf when the leaf represents an intermediate node;
• and
• independently plotting two or more single-cell characteristics in the plurality of single-cell characteristics for the root node of the tree on a third portion of the screen using the corresponding downsampled contiguous blocks representing the two or more single-cell characteristics, across all or the portion of the plurality of bins.