Method for Using a Marker Set for Determining the Risk of Kidney Rejection

A marker set comprising at least three substances chosen from the group consisting of 3-hydroxy isovalerate, acetyl carnitine, alanine, citrate, dimethylamine, glucose, glucuronate, hippurate, lactate, malonate, methyl guanidine, methyl malonate, methyl succinate, p-cresol, phenyl acetate, phenylacetylglycine, phenylacetylglutamine, taurine, trigonelline, and urea, for use in diagnostics of the risk of kidney rejection of an individual after having received a kidney donation, the marker set comprising at least one substance chosen from the group consisting of alanine, dimethylamine, glucuronate, phenylacetylglutamine, and urea.

A method for analyzing an isolated body fluid sample in vitro, comprising the following steps:

• a) providing a body fluid sample from an individual who has received a kidney donation,
• b) determining the concentration of at least three substances chosen from the group consisting of 3-hydroxy isovalerate, acetyl carnitine, alanine, citrate, dimethylamine, glucose, glucuronate, hippurate, lactate, malonate, methyl guanidine, methyl malonate, methyl succinate, p-cresol, phenyl acetate, phenylacetylglycine, phenylacetylglutamine, taurine, trigonelline, and urea in the body fluid sample by analyzing the body fluid sample with a suited measuring technique, with the provision that the concentration of at least one substance chosen from the group consisting of alanine, dimethylamine, glucuronate, phenylacetylglutamine, and urea is determined, and
• c) calculating a score from the determined concentrations, the score being indicative for the risk of kidney rejection of the individual.