Method and device for detecting siglec12
AssigneeThe Regents of the University of California
InventorsAjit Varki, Nissi Varki, Andrea Verhagen, Shoib Siddiqui
Life of this patent
Estimating the term…
Abstract
The present application is in the field of sialic acid biochemistry, metabolism and antigenicity. More particularly, the present invention relates to the detection and analysis of Siglec-XII in a human biological sample for risk prediction, prognostication and diagnosis of disease. Also provided are devices configured to perform the methods disclosed herein.
Claims20
A method for detecting the presence of wild type Siglec-XII in a subject comprising:
- (a) obtaining a sample containing epithelial cells from the subject;
- (b) contacting the sample with a first monoclonal antibody that specifically binds to wild type Siglec-XII;
- and
- (c) detecting the bound first monoclonal antibody,
- thereby detecting the presence of wild type Siglec-XII in the subject.
A method for detecting the severity of cancer in a subject undergoing treatment therefor, the method comprising:
- (a) measuring the level of wild type Siglec-XII in a sample containing epithelial cells from the subject;
- and
- (b) comparing the measured levels against reference levels obtained from a control subject,
- wherein the presence of wild type Siglec-XII in the sample is indicative of late stage progression of the cancer in the subject and the treatment for cancer should be continued.
A kit or article of manufacture comprising:
- (i) reagents specific to detect the presence of wild type Siglec-XII in a biological sample from a subject;
- and (ii) instructions for monitoring progression of cancer in the subject undergoing treatment for cancer or predicting an adverse outcome or risk of an adverse outcome in a subject undergoing a therapeutic regimen for cancer.
Dependencies are inferred from the claim text.