Back to results
US 2020/0003757 A1Published application
Method of identifying agents that affect maturation, survival and myelination
AssigneeKadimastem Ltd.
InventorsMichel Revel, Judith Chebath, Arye Hasson, Michal Izrael, Rosalia Kaufman
Life of this patent
Estimating the term…
Abstract
The present invention discloses a method of identifying agents that affect maturation and survival of oligodendrocytes or myelination of neuronal cells using ex-vivo differentiated embryonic stem cells.
Claims11
-30. (canceled)
A method of making enriched oligodendrocyte precursor cells (OPCs), comprising:
- (a) culturing human embryonic stem cells (hES) in a medium comprising retinoic acid and Epidermal Growth Factor (EGF), thereby generating neurospheres;
- (b) contacting the neurospheres with an adherent substrate comprising an extracellular matrix;
- (c) following step (b), dissociating the neurospheres;
- (d) culturing the dissociated neurospheres in a medium comprising EGF and basic Fibroblast Growth Factor (bFGF);
- (e) following step (d), culturing the dissociated neurospheres in a medium lacking growth factors, thereby generating ex vivo differentiated neural cells;
- (f) selecting the ex vivo differentiated neural cells to be O4-positive or CD140-positive;
- (g) expanding, freezing, and thawing the selected neural cells in a medium comprising EGF and bFGF;
- (h) after thawing, culturing the selected neural cells for at least one day in a medium lacking growth factors;
- thereby making the enriched OPCs.
6 more elements
Dependencies are inferred from the claim text.