Methods of Detecting Lentivirus
OwnerKazuo SUZUKIinventor
Life of this patent
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Abstract
The present disclosure is based on methods for detecting and quantifying lentivirus (human immunodeficiency virus, HIV-1 or HIV-2) DNA and RNA in a biological sample.
Claims29
A method of detecting human immunodeficiency virus (HIV) in a subject with HIV or a subject suspected of having an HIV infection (AIDS), the method comprising performing PCR amplification of R region nucleic acid in a biological sample obtained from the subject, wherein the amplification comprises forward and reverse primers which hybridise to sequences located within the R region of the long terminal repeats (LTRs) of HIV, and subsequently detecting any amplification, wherein detecting amplification is indicative of the presence of HIV in the subject.
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-13. (canceled)
-18. (canceled)
-29. (canceled)
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-42. (canceled)
-48. (canceled)
A composition for amplifying HIV-1 nucleic acid, comprising a labelled oligonucleotide probe comprising or consisting of a combination of probe, forward and reverse primer selected from a combination of one or more of the following:
- (i) oligonucleotide probe:
- SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:45, SEQ ID NO:46, SEQ ID NO:47, or SEQ ID NO:48;
- (ii) forward primer:
- SEQ ID NO:7 or SEQ ID NO:29;
- and
- (iii) reverse primer:
- SEQ ID NO:9 or SEQ ID NO:30.
A composition for amplifying HIV-2 nucleic acid, comprising a labelled oligonucleotide comprising or consisting of a combination of probe, forward and reverse primer selected from a combination of one or more of the following:
- (i) oligonucleotide probe:
- SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:
- 37, SEQ ID NO:
- 38, SEQ ID NO:39, SEQ ID NO:40;
- SEQ ID:41, SEQ ID:42, SEQ ID:43, SEQ ID:44, SEQ ID NO:49, SEQ ID NO:50, SEQ ID NO:51, SEQ ID NO:52 or SEQ ID NO:53;
- (ii) forward primer:
- SEQ ID NO:14, SEQ ID NO:31, SEQ ID NO:33, or SEQ ID NO:35;
- (iii) reverse primer:
- SEQ ID NO:16, SEQ ID NO:32, SEQ ID NO:34 or SEQ ID NO:36.
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Dependencies are inferred from the claim text.