Exosomal nanog dna as a diagnostic cancer marker
AssigneeUniversity of Central Florida Research Foundation, Inc.
InventorsKiminobu SUGAYA, Vaidya MANJUSHA
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Abstract
Disclosed herein are methods of detecting cancer in a patient that involve analyzing DNA in extracellular vesicles. In an exemplary embodiment, detection involves screening for an insert in a 3′ UTR of NANOGP8.
Claims21
A method of screening for cancer in a patient comprising the steps of (i) obtaining a biological sample containing extracellular vesicles from the patient;
- (ii) isolating the vesicles from the biological sample;
- (iii) detecting an amount of NANOG DNA in the vesicles;
- (iv) comparing the amount of NANOG DNA in the vesicles with vesicles in a non-cancer cell sample, wherein an increased level of NANOG DNA in the vesicles from the patient as compared to the non-cancer cell sample provides a positive indication of cancer in the patient.
A method of screening for cancer in a patient comprising the steps of detecting a presence of a 22 bp insert in a NANOGP8 gene or portion thereof in extracellular vesicles from a biological sample from the patient, wherein the 22 bp insert comprises SEQ ID NO. 19 or SEQ ID NO. 20, wherein a presence of the 22 bp insert provides a positive indication of cancer in the patient.
A method of screening for cancer in a patient comprising the steps of detecting a DNA insert in a 3′ UTR of NANOGP8 gene or portion thereof in extracellular vesicles from a biological sample from the patient, wherein a presence of the DNA insert provides a positive indication of cancer in the patient.
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Dependencies are inferred from the claim text.