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AssigneeThe Regents of the University of Michigan
InventorsRonald W. Woodard, Andrew Pratt
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The present invention provides novel, recombinant Gram-negative bacteria. In particular, the invention provides recombinant Gram-negative bacteria (e.g., E. coli) lacking genes involved in lipopolysaccharide (LPS, endotoxin) biosynthesis (e.g., lacking genes required for core oligosaccharide biosynthesis) and also provides recombinant Gram-negative bacteria lacking genes involved in LPS biosynthesis that contain one or more exogenous KDO transferases and/or one or more exogenous heptosyltransferases (e.g., from one or more types and/or strains of bacteria). The invention further provides methods of generating and utilizing (e.g., as or in an immunogenic composition (e.g., as or in an adjuvant and/or vaccine)) the recombinant Gram-negative bacteria therapeutic, preventative, and/or research applications.
A recombinant Gram-negative bacterial cell lacking all endogenous glycosyltransferases involved in core oligosaccharide biosynthesis within lipopolysaccharide (LPS).
A recombinant Gram-negative bacterial cell lacking all endogenous glycosyltransferases involved in core oligosaccharide biosynthesis within lipopolysaccharide (LPS) comprising one or more exogenous KDO transferases.
Lipopolysaccharide (LPS) isolated from a strain of recombinant Gram-negative bacteria genetically modified to:
An immunogenic composition comprising recombinant Gram-negative bacterial cells, or an antigenic component thereof, wherein the recombinant Gram-negative bacterial cells are genetically modified to:
An immunogenic composition comprising lipopolysaccharide (LPS) isolated from a strain of recombinant Gram-negative bacteria genetically modified to:
A method of inducing an immune response in a subject comprising administering to the subject an immunogenic composition comprising recombinant Gram-negative bacterial cells, or an antigenic component thereof, wherein the recombinant Gram-negative bacterial cells are genetically modified to:
Dependencies are inferred from the claim text.