US 2020/0002384 A1 · MUTANT OF ADENO-ASSOCIATED VIRUS (AAV) CAPSID PROTEIN

Abstract

The present invention provides: a mutant of adeno-associated virus (AAV) capsid protein, which contains at least one amino acid substitution in PLA2 domain when compared with the amino acid sequence for wild-type AAV capsid protein; a nucleic acid encoding the mutant; a cell containing the nucleic acid; a method for producing a recombinant AAV particle, comprising a step of culturing the cell to produce the recombinant AAV particle; a recombinant AAV particle containing the mutant; a composition containing the recombinant AAV particle; and a method for transferring a gene into a target cell, comprising a step of bringing the recombinant AAV particle into contact with the target cell.

Claims11

Dependent claims

A mutant of an adeno-associated virus (AAV) capsid protein, comprising one or more amino acid replacements in a PLA2 domain as compared with the amino acid sequence of a wild-type AAV capsid protein,

wherein the one or more amino acid replacements are located at one or more positions selected from the group consisting of:
(1) alanine at position 3,
(2) tyrosine at position 6,
(3) alanine at position 68,
(4) aspartic acid at position 87,
(5) leucine at position 91,
(6) serine at position 149,
(7) proline at position 150, and
(8) serine at position 156
in the amino acid sequence of AAV2 VP1 capsid protein, or at one or more positions corresponding to the above (1) to (8) in the amino acid sequence of VP1 capsid protein of an AAV other than AAV2.

7 more elements

Dependencies are inferred from the claim text.

Facts

Application

16/481,159Filed 29 Jan 2018

Priority

JP 2017-01437730 Jan 2017

Published

2 Jan 2020

Term ends

Not estimatedThe source records don’t support an estimate.

Classification10 codes

C07K 14/005
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof: from viruses
C12N 7/00
Viruses
C12N 15/85
Vectors or expression systems specially adapted for eukaryotic hosts: for animal cells
C12N 2750/14121
Dependovirus, e.g. adenoassociated viruses: viruses as such, e.g. new isolates, mutants or their genomic sequences

Mutant of adeno-associated virus (aav) capsid protein

Life of this patent

Mutant of adeno-associated virus (aav) capsid protein

Life of this patent

Abstract

Claims11