PQQ-sGDH MUTANT, POLYNUCLEOTIDE AND GLUCOSE DETECTION BIOSENSOR
AssigneeLeadway (Hk) Limited
InventorsZhe WANG, Xin BI, Riyong QIU
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Abstract
The present invention provides a soluble pyrroloquinoline-quinone-dependent glucose dehydrogenase (PQQ-sGDH) mutant, wherein the amino acid positions thereof correspond to a wild-type PQQ-sGDH sequence of Acinetobacter calcoaceticus as shown in SEQ ID NO 1 comprising one of the following group of mutations: A194F, a combined mutation based on A194F, a combined mutation based on Q192A or a combined mutation based on Q192S. Said PQQ-sGDH mutant has good glucose substrate specificity and significantly reduced cross-reactivity to maltose and the like, and is suitable for detecting glucose in a sample such as blood.
Claims19
A PQQ-sGDH mutant, said PQQ-sGDH derived from a wild-type PQQ-sGDH amino acid sequence SEQ ID NO 1 of Acinetobacter calcoaceticus, or derived from a wild-type amino acid sequence of other microorganisms having at least 90% homology to SEQ ID NO 1, the numbering of amino acid positions of said PQQ-sGDH mutant corresponding to that of the wild-type PQQ-sGDH amino acid sequence SEQ ID NO 1 from A. calcoaceticus, wherein a mutation carried by said PQQ-sGDH mutant comprises:
- (1) A194F, or (2) one mutation selected from Q192A or Q192S, and said PQQ-sGDH mutant also comprises a substitution mutation occurring at at least one of positions 99, 170, 193, 270, 318, 365, 366, 367, 372, 402, 452 and 455.
Dependencies are inferred from the claim text.