Method for producing n-acetyl-d-glucosamine and/or d-glucosamine salt by microbial fermentation
OwnerLan Suninventor
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Abstract
The present invention discloses a process for producing N-acetyl-D-glucosamine and D-glucosamine salts by microbial fermentation. The invention includes a method to produce N-acetyl-D-glucosamine and/or D-glucosamine salts with higher efficiency and higher yield by increasing the effect of N-acetyl-D-aminomannose-6-phosphate epimerase in microorganisms.
Claims20
A method for producing N-Acetyl-D-Glucosamine and/or D-Glucosamine salt by microbial fermentation, comprising:
- A) culturing a microorganism in a fermentation medium, wherein said microorganism comprises at least one genetic modification that increases the activity of N-acetyl-D-aminomannose-6-phosphate epimerase (NanE) in said microorganism;
- and
- B) collecting N-Acetyl-D-Glucosamine produced in the culturing step A).
A microorganism comprising at least one genetic modification that enhances the activity of N-acetyl-D-aminomannose-6-phosphate epimerase in said microorganism.
An N-acetyl-D-aminomannose-6-phosphate epimerase (NanE) having a higher enzymatic activity, said enzyme having the amino acid sequence of SEQ ID NO.:
- 27.
Dependencies are inferred from the claim text.