Nepetalactol oxidoreductases, nepetalactol synthases, and microbes capable of producing nepetalactone

An expression construct including a nucleotide sequence that encodes an active enzyme, wherein the active enzyme has an amino acid sequence that has at least 70% sequence identity with an amino acid sequence encoded by nucleotide sequence selected from the group consisting of:

• SEQ ID NOs:789-927, wherein the active enzyme is a geraniol diphosphate synthase (GPPS);
• SEQ ID NOs:928-1037, wherein the active enzyme is a geranyl diphosphate diphosphatase (geraniol synthase, GES);
• SEQ ID NOs:1038-1072, 1088-1110, wherein the active enzyme is a geraniol 8-hydroxylase (G8H);
• SEQ ID NOs:1073-1087, wherein the active enzyme is a cytochrome P450 reductase (CPR) capable of promoting regeneration of the redox state of the G8H;
• SEQ ID NOs:1111-1117, wherein the active enzyme is a cytochrome B5 (CYBS) capable of promoting regeneration of the redox state of the G8H;
• SEQ ID NOs:1118-1156, wherein the active enzyme is an 8-hydroxygeraniol dehydrogenase (8HGO);
• SEQ ID NOs:1157-1307, wherein the active enzyme is an iridoid synthase (ISY);
• SEQ ID NOs:1308-1395, 1563-1570, wherein the active enzyme is a nepetalactol oxidoreductase (NOR);
• SEQ ID NOs:1571-1576, wherein the active enzyme is a cytochrome B5 reductase (CYBSR);
• SEQ ID NOs:1506-1562, wherein the active enzyme is a nepetalactol synthase (NEPS);
• SEQ ID NOs:1396-1397, wherein the active enzyme is a GPPS-GES fusion;
• SEQ ID NOs:1398-1462, wherein the active enzyme is a G8H-CPR fusion;
• SEQ ID NOs:1463-1481, wherein the active enzyme is a G8H-CPR-CYBS fusion;
• SEQ ID NOs:1482-1493, wherein the active enzyme is a 8HGO-ISY fusion;
• or SEQ ID NOs:1494-1505, wherein the active enzyme is a ISY-NEPS fusion;
• wherein the enzyme-encoding nucleotide sequence is operably linked to a promoter sequence selected from the group consisting of SEQ ID NOs:1577-1633 or a terminator sequence selected from the group consisting of SEQ ID NOs:1634-1641.