IL-1 Binding Proteins

A binding protein comprising first and second polypeptide chains, wherein said first polypeptide chain comprises a first VD1-(X1)n-VD2-C-(X2)n, wherein:

• VD1 is a first heavy chain variable domain;
• VD2 is a second heavy chain variable domain;
• C is a heavy chain constant domain;
• X1 is a linker with the proviso that it is not CH1;
• X2 is an Fc region;
• and
• n is independently 0 or 1;
• and
• wherein said second polypeptide chain comprises a second VD1-(X1)n-VD2-C-(X2)n,
• wherein:
• VD1 is a first light chain variable domain;
• VD2 is a second light chain variable domain;
• C is a light chain constant domain;
• X1 is a linker with the proviso that it is not CH1;
• X2 does not comprise an Fc region;
• and
• n is independently 0 or 1;
• wherein, in said first polypeptide chain, VD1 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs:
• 213 and 227;
• and VD2 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs:
• 60-148, 196, 198, 200, 202, 204, 206, 208 and 210;
• wherein, in said second polypeptide chain, VD1 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs:
• 216 and 229;
• and VD2 comprises an amino acid sequence selected from the group consisting of SEQ ID NOs:
• 149-189, 197, 199, 201, 203, 205, 207, 209 and 211;
• and wherein the binding protein binds human IL-1β and human IL-1α.

A binding protein comprising an antigen binding domain, said binding protein capable of binding human IL-1β, said antigen binding domain comprising six CDRs:

• CDR-H1, CDR-H2, CDR-H3, CDR-L1, CDR-L2, and CDR-L3, as defined below:
• CDR-H1:
• X1-Y-D-M-S(SEQ ID NO:190), wherein;
• X1 is S, K or R;
• CDR-H2:
• Y-X2-S-X4-G-G-X7-G-T-Y-Y-P-D-X14-X15-K-G (SEQ ID NO:191), wherein;
• X2 is I or V;
• X4 is S or H;
• X7 is G or A;
• X14 is T or S;
• and
• X15 is V or A;
• CDR-H3:
• G-G-V-X4-K-G-X7-F-D-X10 (SEQ ID NO:192), wherein;
• X4 is T or Y;
• X7 is Y or C;
• and
• X10 is V, E, L, M, Q, or Y;
• CDR-L1:
• (SEQ ID NO:193), wherein;
• X7 is H, Y, or W;
• X8 is N, G, T, Q, E, H, D, or K;
• X9 is Y or W;
• and
• X11 is T, A, or N;
• CDR-L2:
• (SEQ ID NO:194), wherein;
• X1 is N, Q, or D;
• X4 is T, N, I, E, or S;
• X6 is A, M, or E;
• and
• X7 is D, E, S, or A;
• and
• CDR-L3:
• Q-X2-F-W-X5-X6-P-X8-X9 (SEQ ID NO:195), wherein;
• X2 is H or Q;
• X5 is S, N, T, K, R, or M;
• X6 is I or L;
• X8 is Y or A;
• and
• X9 is T, I, and N;
• except that when CDR-H1 is S-Y-D-M-S(SEQ ID NO:17), then:
• CDR-H2 cannot be Y-I-S-S-G-G-G-G-T-Y-Y-P-D-T-V-K-G (SEQ ID NO:18);
• CDR-H3 cannot be G-G-V-T-K-G-Y-F-D-V (SEQ ID NO:19);
• CDR-L1 cannot be R-A-S-G-N-I-H-N-Y-L-T (SEQ ID NO:20);
• CDR-L2 cannot be N-A-K-T-L-A-D (SEQ ID NO:21);
• and
• CDR-L3 cannot be Q-H-F-W-S-I-P-Y-T (SEQ ID NO:22).