A genetically modified bacterial cell factory for thiamine production

A genetically modified bacterium for production of un-phosphorylated thiamine;

• wherein said bacterium is characterized by having transgenes encoding:
• a. a polypeptide having thiamine mono-phosphate phosphatase activity (E.C. 3.1.3.-), wherein the amino acid sequence of said polypeptide has at least 80% sequence identity to a sequence selected from the group consisting of SEQ ID No:
• 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72 and 74;
• b. a polypeptide having 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate (HMP-P) synthase activity (E.C. 4.1.99.17);
• c. a polypeptide having thiamine phosphate synthase activity (E.C.2.5.1.3);
• d. a polypeptide having ThiS adenylyltransferase activity (E.C.2.7.7.73);
• e. a polypeptide having ThiS sulfur-carrier activity;
• f. a polypeptide having thiazole synthase activity (E.C.2.8.1.10);
• g. a polypeptide having 2-iminoacetate synthase activity (4.1.99.19) or having Glycine oxidase activity (EC 1.4.3.19);
• and
• h. a polypeptide having phosphohydroxymethylpyrimidine kinase activity (E.C.2.7.4.7), wherein the genus of the bacterium is Escherichia.