Synthesis of functionalised gold nanoparticles and nanocompounds containing same for measuring sucrose or starch in cells

Method for the measurement of sucrose or starch in cells which comprises the steps of:

• a) Synthesizing gold nanoparticles in solution with citric acid;
• b) Functionalizing the synthesized gold nanoparticles (a) with the polymeric ligands SH(CH2)12COOH;
• c) Adding half a portion of the functionalized AuNPs nanoparticle solution obtained in step b) to a cytosol solution;
• d) Resting until achieving thermal equilibrium of the solution obtained in c);
• e) Adding fluorescein to the remaining half portion of solution of functionalized AuNPs obtained in c);
• f) Adding the same concentration of fluorescein obtained in step e) to the half portion of solution of AuNPs functionalized in cytosol, obtained in step d);
• g) Calculating the difference of the fluorescence intensity measurement between the solution of AuNPs functionalized in e) and the fluorescein solution in f).