Method for producing nucleic acid array and device for producing nucleic acid array
AssigneeNikon Corporation
InventorYusuke KAWAKAMI
Life of this patent
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Abstract
A method for producing a nucleic acid array which includes (a) a step of forming a layer (a PAG layer) made of a resin composition containing a photoacid generator (PAG) for generating an acid as a result of being exposed to light on a solid phase which has a molecule immobilized thereon and having a functional group protected by an acid-decomposable protective group; (b) a step of exposing a desired position of the PAG layer to light; (c) a step of removing the PAG layer which has been exposed to light; and (d) a step of bringing the solid phase from which the PAG layer has been removed into contact with a nucleotide derivative having an acid-decomposable protective group is provided.
Claims12
A method for producing a nucleic acid array, comprising:
- (a) a step of forming a layer (a PAG layer) made of a resin composition containing a photo acid generator (PAG) for generating an acid as a result of being exposed to light on a solid phase which has a molecule immobilized thereon and having a functional group protected by an acid-decomposable protective group;
- (b) a step of exposing a desired position of the PAG layer to light;
- (c) a step of removing the PAG layer from which has been exposed to light;
- and
- (d) a step of bringing the solid phase from which the PAG layer has been removed into contact with a nucleotide derivative having an acid-decomposable protective group.
A nucleic acid array production device comprising:
- a PAG layer formation part which is configured to form a layer (a PAG layer) made of a resin composition containing a PAG for generating an acid as a result of being exposed to light on a solid phase which has a molecule immobilized thereon and having a functional group protected by an acid-decomposable protective group;
- a light exposure part which is configured to expose a desired position of the PAG layer to light;
- a PAG layer removal part which is configured to remove the PAG layer which has been exposed to light;
- and
- a nucleotide derivative reaction part which is configured to bring the solid phase from which the PAG layer has been removed into contact with a nucleotide derivative having an acid-decomposable protective group.
Dependencies are inferred from the claim text.