# US 2019/0376952 A1 · Image processing device, analysis device, image processing method, image processing program, and display device

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- Number: US 2019/0376952 A1
- Document ID: US20190376952A1
- Status: Published application
- Kind: A1
- Application number: 16/481,803
- Filed: 2017-02-03
- Published: 2019-12-12
- Earliest claimed: 2017-02-03
- Assignees: Nikon Corporation
- Applicants: Nikon Corporation
- Inventors: Kenta IMAI; Mamiko MASUTANI
- Web page: https://patentgrep.com/patent/US20190376952A1

## CPC

- C12Q 1/02: involving viable microorganisms
- G01N 33/5044: {involving specific cell types}
- C12M 1/00: Apparatus for enzymology or microbiology
- C12M 1/34: Measuring or testing with condition measuring or sensing means, e.g. colony counters
- G06T 7/00: Image analysis
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS}
- G06T: IMAGE DATA PROCESSING OR GENERATION, IN GENERAL

## Abstract

An image processing device includes a determination unit configured to determine a type of autophagy induced in a cell, based on information indicative of autophagic activity in the cell present in a cell image in which the cell is image captured and based on information indicative of congestion of molecules in the cell present in the cell image.

## Claims

### Claim 1

An image processing device comprising
a determination unit configured to determine a type of autophagy induced in a cell, based on information indicative of autophagic activity in the cell present in a cell image in which the cell is image captured and based on information indicative of congestion of molecules in the cell present in the cell image.

### Claim 2 (depends on 1)

The image processing device according to claim 1, wherein
the type of autophagy induced in the cell includes a selective autophagy and a non-selective autophagy, and
the determination unit determines whether the autophagy induced in the cell is a selective autophagy or a non-selective autophagy, based on information indicative of a degree of autophagic activity in the cell present in the cell image and based on information indicative of a degree of congestion of molecules in the cell.

### Claim 3 (depends on 2)

The image processing device according to claim 2, wherein
the cell includes a stimulated cell, and
a stimulus determination unit configured to determine whether the stimulus induces a selective autophagy or a non-selective autophagy, based on the determination by the determination unit is further provided.

### Claim 4 (depends on 1)

The image processing device according to claim 1,
further including a cell image acquisition unit configured to acquire a cell image in which the cell is image captured.

### Claim 5 (depends on 4)

The image processing device according to claim 4, wherein
the cell includes a stimulated cell,
the cell image acquisition unit acquires a plurality of cell images in which the stimulated cell is image captured, and
the image processing device comprising
a feature value calculation unit configured to calculate feature values of subcellular components, based on a cell image acquired by the cell image acquisition unit, and
a correlation calculation unit configured to calculate a correlation between the feature values of the subcellular components.

### Claim 6 (depends on 5)

The image processing device of claim 5, further comprising
a correlation comparison unit configured to perform a comparison of a correlation between feature values of subcellular components calculated based on a cell image of the stimulated cell with a correlation between feature values of subcellular components calculated based on a cell image of the cell having not received the stimulus.

### Claim 7 (depends on 6)

The image processing device according to claim 6, further comprising
a molecule identification unit configured to identify a molecule involved in the autophagy by using a result of the correlation comparison unit.

### Claim 8 (depends on 7)

The image processing device according to claim 7, wherein
a feature value of a subcellular component includes a degree of activity of the autophagy and a degree of congestion of the molecules, and
the molecule identification unit calculates a correlation including a degree of activity of the autophagy and a degree of congestion of the molecules.

### Claim 9 (depends on 8)

The image processing device according to claim 8, wherein
the molecule identification unit identifies a molecule involved in the autophagy, based on the correlation including the degree of activity of the autophagy and the degree of congestion of the molecules.

### Claim 10 (depends on 2)

The image processing device according to claim 2, wherein
the degree of activity of the autophagy includes number of autophagosomes.

### Claim 11 (depends on 1)

The image processing device according to claim 1, wherein
the degree of congestion of the molecules includes molecular congestion.

### Claim 12 (depends on 2)

The image processing device according to claim 2, wherein
information indicative of the degree of activity of the autophagy and/or information indicative of the congestion of molecules in the cell is analyzed based on information different from the cell image, and the analyzed result is further used to determine whether the selective autophagy or the non-selective autophagy is applicable.

### Claim 13 (depends on 1)

The image processing device according to claim 1, further comprising
a display unit configured to display a determination result of the determination unit.

### Claim 14

An analysis device comprising:
a feature value calculation unit configured to calculate a feature value of a subcellular component, based on a cell image in which the cell is image captured;
a function estimation unit configured to estimate a function of the cell; and
a function correlation calculation unit configured to calculate a correlation between the function of the cell estimated by the function estimation unit and the feature value of the subcellular component.

### Claim 15 (depends on 14)

The analysis device of claim 14, wherein
the function estimated by the function estimation unit includes a type of autophagy induced in the cell.

### Claim 16 (depends on 15)

The analysis device according to claim 15, wherein
the type of autophagy is determined based on information indicative of autophagic activity and information indicative of congestion of molecules in the cell,
the correlation between the function of the cell and the feature value of the subcellular component includes at least one of a correlation between the information indicative of autophagic activity and the feature value of the subcellular component; and a correlation between information indicative of congestion of molecules in the cell and the feature value of the subcellular component.

### Claim 17 (depends on 14)

The analysis device according to claim 14, wherein
the type of autophagy induced in the cell includes either a selective autophagy or a non-selective autophagy.

### Claim 18

An image processing method comprising
a determining step of determining a type of autophagy induced in a cell, based on information indicative of autophagic activity in the cell present in a cell image in which the cell is image captured and based on information indicative of congestion of molecules in the cell present in the cell image.

### Claim 19

An image processing program causing a computer to execute
a determining step of determining a type of autophagy induced in a cell, based on information indicative of autophagic activity in the cell present in a cell image in which the cell is image captured and based on information indicative of congestion of molecules in the cell present in the cell image.

### Claim 20

A display device comprising
a display unit configured to display a determination result acquired from a determination unit configured to determine a type of autophagy induced in a cell, based on information indicative of autophagic activity in the cell present in a cell image in which the cell is image captured and based on information indicative of congestion of molecules in the cell present in the cell image.