# US 2019/0376104 A1 · Production method for useful substance > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2019/0376104 A1 - Document ID: US20190376104A1 - Status: Published application - Kind: A1 - Application number: 16/486,011 - Filed: 2018-02-28 - Published: 2019-12-12 - Earliest claimed: 2017-03-01 - Assignees: Sanyo Chemical Industries, Ltd. - Applicants: Sanyo Chemical Industries, Ltd. - Inventors: Masumi UEDA; Mutsumi NAKANISHI; Yasunori CHIBA; Takehiko YOKO-O - Web page: https://patentgrep.com/patent/US20190376104A1 ## CPC - C12P 1/02: by using fungi - C12P 21/02: having a known sequence of two or more amino acids, e.g. glutathione - C12N 9/0069: {acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)} - C12P 21/00: Preparation of peptides or proteins (single cell protein C12N1/00) - C12Y 113/12: with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12) - C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)} - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - C12Y: ENZYMES ## Abstract The present invention provides a method, of producing a useful substance, including secreting and producing a useful substance in broth by yeast contained in the broth, the yeast being a Saccharomyces strain, the broth containing a compound (A) represented by a formula (1), the compound (A) having an HLB value of 0.1 to 16 and a number average molecular weight of 200 to 30000, the weight percentage of the compound (A) based on the weight of the broth being 0.0001 to 10 wt %: HO—(A1O)m1-(A2O)m2-(A3O) m3—H (1) wherein A1O, A2O, and A3O each independently represent a C2-C4 oxyalkylene group; A1O and A2O have different structural formulas, A2O and A3O have different structural formulas; and m1, m2, and m3 each respectively represent the average number of moles of A1O, A2O, and A3O added, and are each independently a number in the range of 1 to 600. ## Claims ### Claim 1 A method of producing a useful substance, comprising: secreting and producing a useful substance in broth by yeast contained in the broth, the yeast being a Saccharomyces strain, the broth containing a compound (A) represented by a formula (1), the compound (A) having an HLB value of 0.1 to 16 and a number average molecular weight of 200 to 30000, the weight percentage of the compound (A) based on the weight of the broth being 0.0001 to 10 wt %: HO—(A1O)m1-(A2O)m2-(A3O) m3—H (1) wherein A1O, A2O, and A3O each independently represent a C2-C4 oxyalkylene group; A1O and A2O have different structural formulas, A2O and A3O have different structural formulas; and m1, m2, and m3 each respectively represent the average number of moles of A1O, A2O, and A3O added, and are each independently a number in the range of 1 to 600. ### Claim 2 (depends on 1) The method of producing a useful substance according to claim 1, wherein the useful substance is a protein. ### Claim 3 (depends on 1) The method of producing a useful substance according to claim 1, wherein A1O, A2O, and A3O in the compound (A) represented by the formula (1) each independently represent an oxyethylene group or a 1,2-oxypropylene group. ### Claim 4 (depends on 1) The method of producing a useful substance according to claim 1, wherein A1O and A3O are oxyethylene groups and A2O is a 1,2-oxypropylene group in the compound (A) represented by the formula (1). ### Claim 5 (depends on 1) The method of producing a useful substance according to claim 1, wherein the compound (A) represented by the formula (1) has a number average molecular weight of 1000 to 20000, A2O is a 1,2-oxypropylene group, and m2 is a number in the range of 10 to 70. ### Claim 6 (depends on 2) The method of producing a useful substance according to claim 2, wherein A1O, A2O, and A3O in the compound (A) represented by the formula (1) each independently represent an oxyethylene group or a 1,2-oxypropylene group. ### Claim 7 (depends on 2) The method of producing a useful substance according to claim 2, wherein A1O and A3O are oxyethylene groups and A2O is a 1,2-oxypropylene group in the compound (A) represented by the formula (1). ### Claim 8 (depends on 3) The method of producing a useful substance according to claim 3, wherein A1O and A3O are oxyethylene groups and A2O is a 1,2-oxypropylene group in the compound (A) represented by the formula (1). ### Claim 9 (depends on 6) The method of producing a useful substance according to claim 6, wherein A1O and A3O are oxyethylene groups and A2O is a 1,2-oxypropylene group in the compound (A) represented by the formula (1). ### Claim 10 (depends on 2) The method of producing a useful substance according to claim 2, wherein the compound (A) represented by the formula (1) has a number average molecular weight of 1000 to 20000, A2O is a 1,2-oxypropylene group, and m2 is a number in the range of 10 to 70. ### Claim 11 (depends on 3) The method of producing a useful substance according to claim 3, wherein the compound (A) represented by the formula (1) has a number average molecular weight of 1000 to 20000, A2O is a 1,2-oxypropylene group, and m2 is a number in the range of 10 to 70. ### Claim 12 (depends on 6) The method of producing a useful substance according to claim 6, wherein the compound (A) represented by the formula (1) has a number average molecular weight of 1000 to 20000, A2O is a 1,2-oxypropylene group, and m2 is a number in the range of 10 to 70. ### Claim 13 (depends on 4) The method of producing a useful substance according to claim 4, wherein the compound (A) represented by the formula (1) has a number average molecular weight of 1000 to 20000, A2O is a 1,2-oxypropylene group, and m2 is a number in the range of 10 to 70. ### Claim 14 (depends on 7) The method of producing a useful substance according to claim 7, wherein the compound (A) represented by the formula (1) has a number average molecular weight of 1000 to 20000, A2O is a 1,2-oxypropylene group, and m2 is a number in the range of 10 to 70. ### Claim 15 (depends on 8) The method of producing a useful substance according to claim 8, wherein the compound (A) represented by the formula (1) has a number average molecular weight of 1000 to 20000, A2O is a 1,2-oxypropylene group, and m2 is a number in the range of 10 to 70. ### Claim 16 (depends on 9) The method of producing a useful substance according to claim 9, wherein the compound (A) represented by the formula (1) has a number average molecular weight of 1000 to 20000, A2O is a 1,2-oxypropylene group, and m2 is a number in the range of 10 to 70.