# US 2019/0376017 A1 · Process for providing a culture of microorganisms to an elongated element > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2019/0376017 A1 - Document ID: US20190376017A1 - Status: Published application - Kind: A1 - Application number: 16/436,046 - Filed: 2019-06-10 - Published: 2019-12-12 - Earliest claimed: 2018-06-12 - Assignees: Sanko Tekstil Isletmeleri San. Ve Tic. A.S. - Applicants: Sanko Tekstil Isletmeleri San. Ve Tic. A.S. - Inventors: Jitka ERYILMAZ; Ece SENEL; Seref AGZIKARA; Nejdiye GUNES; Fehim CAGLAR; Semih KAZANC; Ozgur COBANOGLU - Web page: https://patentgrep.com/patent/US20190376017A1 ## CPC - C12M 33/04: {by injection or suction, e.g. using pipettes, syringes, needles (pipettes in general B01L3/02)} - C12M 23/06: {Tubular (C12M23/08, C12M23/16 take precedence)} - C12M 41/14: {Incubators; Climatic chambers (per se B01L1/00)} - C12M 41/36: {of biomass, e.g. colony counters or by turbidity measurements (electrooptical investigation of individual particles G01N15/14, flow cytometers G01N15/1404)} - C12M 41/48: {Automatic or computerized control (automatic analysis G01N35/00)} - C12N 1/00: Microorganisms; Compositions thereof (medicinal preparations containing material from protozoa, bacteria or viruses A61K35/66, from algae A61K36/02, from fungi A61K36/06; preparing medicinal bacterial antigen or antibody compositions, e.g. bacterial vaccines, A61K39/00); Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor - C12N 1/12: Unicellular algae; Culture media therefor (as new plants A01H13/00) - C12N 1/14: Fungi (culture of mushrooms A01G18/00; as new plants A01H15/00); Culture media therefor - C12N 1/16: Yeasts; Culture media therefor - C12N 1/20: Bacteria; Culture media therefor - C12P 3/00: Preparation of elements or inorganic compounds except carbon dioxide {(recovery of carbon dioxides as by-products C12F3/02)} - C12P 19/04: Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds - D06B 1/08: from outlets being in, or almost in, contact with the textile material - D06M 16/003: {with enzymes or microorganisms} - D06M 23/02: Processes in which the treating agent is releasably affixed or incorporated into a dispensing means - C12N 2527/00: Culture process characterised by the use of mechanical forces, e.g. strain, vibration - C12N 2533/00: Supports or coatings for cell culture, characterised by material - C12R 2001/00: Microorganisms {; Processes using microorganisms} - D06B 5/06: through yarns, threads or filaments - D06M 2101/06: cellulosic - C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS} - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)} - D06B: TREATING TEXTILE MATERIALS USING LIQUIDS, GASES OR VAPOURS (mechanical treatment of natural fibrous or filamentary material to obtain fibres or filaments D01B) - D06M: TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS - C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS ## Abstract The present invention relates to a process for depositing at least a culture of microorganisms to an elongated element, preferably a yarn, comprising the steps of: providing at least a first feeding device comprising at least a first outlet; supplying at least one elongated element to said at least first feeding device; feeding to said first outlet at least a first culture comprising at least one microorganism; dispensing said first culture from said at least first outlet; contacting at least part of said elongated element with said first culture of microorganisms, to provide at least a part of said elongated element with an amount of said first culture of microorganisms. ## Claims ### Claim 1 A process for depositing at least a culture (5) of microorganisms to an elongated element (2), comprising the steps of: a) providing at least a first feeding device (4) comprising at least a first outlet (7); b) supplying at least one elongated element(2) to said at least first feeding device (4); c) feeding to said first outlet (7) at least a first culture (5) comprising at least one microorganism; d) dispensing said first culture (5) from said at least first outlet (7); e) contacting at least part of said elongated element (2) with said first culture (5) of microorganisms, to provide at least a part of said elongated element (2) with an amount of said first culture (5) of microorganisms. ### Claim 2 (depends on 1) The process according to claim 1, wherein said first culture (5) of microorganisms is in liquid form and is dispensed from said first outlet (7) to said elongated element (2) discontinuously or continuously. ### Claim 3 (depends on 1) The process according to claim 1, wherein said elongated element (2) provided with said first culture (5) of microorganisms is collected at an elongated element take-up device (3). ### Claim 4 (depends on 1) The process according to claim 1, wherein said first culture (5) is dispensed to said elongated element (2) according to a direction that is substantially perpendicular to the direction of supply of said elongated element (2). ### Claim 5 (depends on 1) The process according to claim 1, wherein said elongated element (2) is fed to said outlet (7) at a distance whereby said culture (5) exiting said outlet (7) contacts both said outlet (7) and said elongated element (2) during said elongated element contacting step. ### Claim 6 (depends on 5) The process according to claim 5, wherein said distance is in the range of 0.1 mm to 5 mm. ### Claim 7 (depends on 1) The process according to claim 1, comprising the step of selectively varying the speed of said dispensing of said first culture (5) in function of the speed of supply of said elongated element (2), and/or as a function of the absorption capacity, and/or the dimension of said at least one elongated element (2). ### Claim 8 (depends on 1) The process according to claim 1, further comprising a step f) of incubating the elongated element obtained in said step e). ### Claim 9 (depends on 8) The process according to claim 8, wherein the elongated element obtained in said step e) is incubated in air and/or in an incubator (9). ### Claim 10 (depends on 1) The process according to claim 1, wherein at least a second outlet (17) is provided, preferably downstream said first outlet (7, 16), wherein said at least second outlet (17) dispenses a second culture of microorganisms in liquid form or a culture medium. ### Claim 11 (depends on 1) The process according to claim 1, wherein at least said first culture (5) and/or culture medium is dispensed to said elongated element (2) within said incubator (9). ### Claim 12 (depends on 1) The process according to claim 1, wherein said culture (5) of microorganisms comprises at least one microorganism selected from bacteria, yeasts, fungi, algae and mixtures thereof. ### Claim 13 (depends on 1) The process according to claim 1, wherein said culture (5) of microorganisms comprises at least one microorganism able to provide a microbial product and/or a microbial precipitate onto the elongated element (2). ### Claim 14 (depends on 13) The process according to claim 13, wherein said microbial product is a biopolymer and/or an enzyme, and/or a dye, wherein said microbial product is selected from a sugar-based biopolymer, an amino acid-based biopolymer, and urease and wherein said dye is selected from indigo dye, indigoid dye, pigment dye and mixture thereof. ### Claim 15 (depends on 13) The process according to claim 13, wherein said microbial precipitate is calcite precipitate. ### Claim 16 (depends on 1) The process according to claim 1, wherein said culture comprises microorganisms at a concentration 1×108 CFU/ml to 1×109 CFU/ml. ### Claim 17 (depends on 1) The process according to claim 1, wherein said elongated element (2) is a yarn (2). ### Claim 18 (depends on 1) The process according to claim 1, wherein a wetting agent is fed to said elongated element, in an amount in the range of 0.05% to 1% by weight of the final culture weight. ### Claim 19 (depends on 1) An elongated element obtainable by the process of claim 1, wherein at least part of said elongated element is provided with a microbial product, and/or a microbial precipitate. ### Claim 20 (depends on 19) The elongated element according to claim 19, wherein said elongated element is dry and optionally dyed. ### Claim 21 (depends on 1) An apparatus for carrying out the process according to claim 1 comprising at least a first feeding device (4) having at least one first outlet (7) for dispensing at least a first culture (5) comprising at least one microorganism from said outlet (7), and at least one first elongated element source (1) to supply at least one elongated element (2) to said device, wherein said apparatus is configured so that said at least a first culture (5) comprising at least one microorganism contacts at least part of said elongated element (2) when said culture is dispensed from said first outlet (7). ### Claim 22 (depends on 21) The apparatus according to claim 21, further comprising at least one incubator (9). ### Claim 23 (depends on 21) The apparatus according to claim 21, further comprising at least a second feeding device (13) having at least one second outlet for dispensing a culture of microorganisms or a culture medium. ### Claim 24 (depends on 22) The apparatus according to claim 22, wherein said at least one incubator (9) includes said first feeding device (4), and/or said second feeding device (13), and/or at least one elongated element take-up device (3), wherein said elongated element take-up device (3) is configured to collect said elongated element after it has been provided with said culture (5). ### Claim 25 (depends on 21) The apparatus according to claim 21, further comprising a logic control unit configured to regulate the speed of dispensing of at least said first culture and/or the speed of supplying of said elongated element, and further comprising one or more sensors for monitoring culture at the outlet. ### Claim 26 (depends on 21) The apparatus according to claim 21, wherein said feeding device (4) comprise a pump (15).